Anti-PIGX Rabbit Polyclonal Antibody for WB, ELISA - Q8TBF5

Core Product Specifications and Parameters

Biological Background: PIGX Function and Localization

• PIGX (Phosphatidylinositol-glycan biosynthesis class X protein) is the stabilizing subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex, essential for GPI-anchor biosynthesis.
• It facilitates the transfer of the first mannose residue via an α-1,4 linkage from dolichol-phosphate-mannose (Dol-P-Man) to the GlcN-(acyl)PI intermediate, a critical sixth step in the GPI-anchor pathway.
• PIGX likely acts by stabilizing the catalytic mannosyltransferase subunit PIGM, ensuring proper complex function at the endoplasmic reticulum membrane.
• Subcellular localization is restricted to the endoplasmic reticulum membrane, where GPI-anchor assembly occurs.
• The protein contains a signal peptide and a transmembrane helix, classifying it as a single-pass membrane glycoprotein.
• Alternative splicing of the PIGX gene yields multiple isoforms, contributing to regulatory diversity in GPI biosynthesis.
• Keywords from UniProt highlight its role in GPI-anchor biosynthesis, glycoprotein metabolism, and its identification via proteomics in the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 80–220 of human PIGX, a region that may present suitable epitopes; consider this in assay design.
• For western blotting, the expected molecular weight is approximately 29 kDa; validate detection in the relevant sample system under optimized conditions.
• This antibody has reported cross-reactivity with rat; thus, it may be suitable for studies involving rat-derived samples, though species-specific validation is advised.
• ELISA applications can be explored with appropriate coating and detection protocols; empirical optimization of antibody concentration is recommended.
• When planning experiments, consider the low endogenous expression of PIGX and the need for enrichment or sensitive detection methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a comprehensive one-stop source for IVD raw materials, including this PIGX rabbit polyclonal antibody for GPI-anchor biosynthesis research. Beyond this reagent, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials—supporting your entire development from concept to clinic.