Anti-PIGR Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P01833

Core Product Specifications and Parameters

Biological Background: PIGR Function and Localization

• Polymeric immunoglobulin receptor (PIGR), also known as hepatocellular carcinoma-associated protein TB6, is a transmembrane glycoprotein central to mucosal immune defense.
• It mediates selective transcytosis of polymeric IgA (pIgA) and IgM across mucosal epithelial cells, binding them at the basolateral surface and transporting the complex to the apical surface for secretion.
• During transcytosis, the extracellular ligand-binding domain (secretory component) is proteolytically cleaved and released with the immunoglobulin, forming secretory IgA (sIgA) that is anchored to the mucus lining via N-linked glycans. Related references: PMID:12150896
• In its free form (secretory component), PIGR can act as a non-specific microbial scavenger, directly binding to bacteria and preventing their adhesion to epithelial cells. Related references: PMID:16543244
• PIGR localizes to the cell membrane and is also found secreted after cleavage; its subcellular distribution reflects its transcytotic and luminal release functions.
• The protein features immunoglobulin-like domains, multiple disulfide bonds, glycosylation sites, and known phosphorylation, as highlighted by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 460–600 of human PIGR, a region within the extracellular secretory component; this design likely ensures recognition of both full-length receptor and cleaved secretory component in denaturing and native assays.
• For Western blot, the predicted full-length molecular weight is ~83 kDa; cleavage products (~80 kDa) may also appear. Consider testing reducing and non-reducing conditions due to the presence of disulfide bonds.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), expect staining at the apical surface of mucosal epithelial cells and in secreted material; include appropriate tissue controls.
• Cross-reactivity with mouse and rat has been confirmed; for other species, verify sequence homology before use.
• For ELISA applications, this antibody may serve as a detection reagent; pairing with a suitable capture antibody should be optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibodies, antigens, and ancillary reagents to diagnostic manufacturers and research laboratories worldwide. This anti-PIGR antibody supports studies in mucosal immunology, infectious disease, and diagnostic assay development. Contact us for bulk orders, custom preparations, or complementary reagents to accelerate your project from concept to clinic.