Anti-PIGQ Rabbit Polyclonal Antibody for WB, ELISA - Q9BRB3

Core Product Specifications and Parameters

Biological Background: PIGQ Function and Localization

• PIGQ (Phosphatidylinositol N-acetylglucosaminyltransferase subunit Q), also known as GPI1 or PIG-Q, is a subunit of the GPI-GnT complex.
• The GPI-GnT complex catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step in GPI anchor biosynthesis.
• GPI anchors are glycolipids that attach many proteins to the cell surface, essential for normal cellular function.
• Mutations in PIGQ are associated with diseases such as developmental and epileptic encephalopathy 77 (DEE77) and multiple congenital anomalies-hypotonia-seizures syndrome 4 (MCAHS4).
• PIGQ localizes to the membrane and contains transmembrane helices, consistent with its role in GPI synthesis at the endoplasmic reticulum.
• Alternative splicing generates multiple isoforms; the canonical isoform has a molecular weight of approximately 84 kDa.
• Keywords: Alternative splicing, Disease variant, Epilepsy, GPI-anchor biosynthesis, Membrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-200) of human PIGQ, which may be conserved across species; cross-reactivity with mouse is observed.
• For Western blotting, consider a starting dilution range based on endogenous expression levels in relevant cell lines; the predicted molecular weight is ~84 kDa.
• The antibody may also be suitable for ELISA; optimize coating and detection conditions in the target sample matrix.
• As PIGQ is a multi-pass membrane protein, consider including mild detergents in lysis buffers to maintain solubility.
• Validate specificity using knockout or knockdown controls if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated antibodies for GPI biosynthesis research, such as this anti-PIGQ polyclonal antibody, ideal for Western blot and ELISA applications. We support your workflow with bulk ancillary reagents, optimized antibody pairs, and rare target sourcing to accelerate your IVD development from concept to clinic.