Anti-PIGP Rabbit Polyclonal Antibody for WB and ELISA - P57054

Core Product Specifications and Parameters

Biological Background: PIGP Function and Localization

• PIGP, also known as phosphatidylinositol N-acetylglucosaminyltransferase subunit P, Down syndrome critical region protein 5 (DSCR5), and DCRC, is encoded by the PIGP gene.
• It is a component of the glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex, which catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol—the first step in GPI anchor biosynthesis.
• As a transmembrane protein, PIGP localizes to cellular membranes, where it participates in the early stages of GPI anchor assembly.
• Expression of PIGP is ubiquitous across human tissues, reflecting its fundamental role in basic cellular processes.
• The protein belongs to the PIGP family and is predicted to have transmembrane helices; its canonical isoform has a molecular weight of approximately 18 kDa.
• Mutations in PIGP are associated with developmental and epileptic encephalopathy type 55 (DEE55), underscoring its importance in neurodevelopment.
• Alternative splicing generates multiple isoforms, and the protein undergoes proteomics identification; no specific post-translational modifications are annotated in UniProt.
• Keywords: GPI-anchor biosynthesis, membrane, transmembrane helix, epilepsy, disease variant, alternative splicing.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is 18 kDa; however, due to alternative splicing or potential post-translational modifications, multiple bands may be observed. Validate using appropriate controls.
• This polyclonal antibody was raised against a recombinant immunogen (aa 25-158) and is suitable for both WB and ELISA. Consider optimizing dilution factors (e.g., starting at 1:500–1:2000 for WB, 1:1000–1:5000 for ELISA) based on your experimental system.
• Cross-reactivity with mouse has been confirmed; when testing other species, validate reactivity in the relevant sample system.
• As PIGP is a membrane protein, enriching membrane fractions may improve detection sensitivity in WB. Also, include loading controls (e.g., GAPDH, β-actin) and negative controls such as PIGP knockout lysates if available.
• For ELISA applications, consider using the antibody in a sandwich or direct assay with a recombinant PIGP protein or peptide as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PIGP polyclonal antibody supports research into GPI biosynthesis and associated disorders, and we offer a full suite of complementary raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or emerging targets like PIGP, our sourcing expertise ensures you get the critical components needed for your assay development.