Anti-PIGO Polyclonal Antibody for WB and ELISA - Q8TEQ8

Core Product Specifications and Parameters

Biological Background: PIGO Function and Localization

• PIGO (Phosphatidylinositol-glycan biosynthesis class O protein) is the catalytic subunit of the GPI ethanolamine phosphate transferase 3 complex, also known as PIG-O or hGPCR43.
• It encodes a transmembrane protein that catalyzes the tenth step of glycosylphosphatidylinositol (GPI)-anchor biosynthesis by transferring ethanolamine phosphate from phosphatidylethanolamine to the third mannose of the GPI intermediate.
• The protein localizes to the endoplasmic reticulum membrane, consistent with its role in early GPI anchor assembly.
• PIGO is classified as a transferase and contains predicted transmembrane helices, indicating integration into the ER membrane.
• Alternative splicing gives rise to transcript variants, potentially influencing isoform function or regulation.
• Mutations in PIGO are associated with disease variants linked to intellectual disability, underscoring its importance in neurodevelopment.
• As a key enzyme in GPI biosynthesis, PIGO is essential for the attachment of proteins to the cell surface, impacting signal transduction and cell adhesion.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 950–1050 of human PIGO; this C‑terminal region may be suitable for detecting full‑length protein in western blotting.
• For WB, a band around 119 kDa may be observed; consider using appropriate positive controls such as human or mouse cell lines that express PIGO.
• When performing ELISA, verify coating and detection conditions with the relevant samples, as polyclonal antibodies may exhibit batch‑to‑batch variation.
• Cross‑reactivity is reported for human and mouse; for other species, validation in the target sample system is recommended before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies, to support diagnostic manufacturers and research laboratories from concept to clinic. This anti‑PIGO polyclonal antibody is designed for applications such as WB and ELISA, making it a valuable tool for studying GPI‑anchor biosynthesis and related disorders. Through our sourcing network, we can also provide bulk ancillary reagents and access to rare target raw materials to accelerate your assay development workflows.