Anti-PIGM Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9H3S5

Core Product Specifications and Parameters

Biological Background: PIGM Function and Localization

• PIGM is the catalytic subunit of the GPI-mannosyltransferase I complex, responsible for transferring the first mannose to the GPI anchor intermediate via an alpha-1,4 linkage.
• It plays an essential role in the sixth step of GPI-anchor biosynthesis, a critical process for anchoring proteins to the cell membrane.
• The enzyme utilizes dolichol-phosphate-mannose (Dol-P-Man) as a mannose donor.
• PIGM localizes to the endoplasmic reticulum membrane, consistent with its role in GPI anchor assembly.
• Encoded by the PIGM gene, it belongs to the glycosyltransferase family involved in phosphatidylinositol-glycan biosynthesis.
• Listed as a multi-pass transmembrane protein with transferase activity, as per UniProt annotations.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 35-96) of human PIGM; verify sequence identity across species for cross-reactivity predictions.
• For Western blot, a band around 49 kDa is expected; optimize protein loading and lysis conditions for membrane proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), include appropriate permeabilization steps to access the ER membrane-localized target.
• ELISA performance may depend on coating conditions and detection format; consider using recombinant proteins as standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-PIGM antibody, targeting a key enzyme in GPI-anchor biosynthesis, supports research in cell membrane biology and related disorders. For custom or bulk procurement needs, contact our team for dedicated support.