Anti-Pig CD27 Monoclonal Antibody for FC - L7QHB1

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 is a costimulatory immune-checkpoint receptor expressed on the surface of T-cells, NK-cells, and B-cells.
• It binds to its ligand CD70 (CD27L) expressed on B-cells, mediating antigen-specific T-cell activation and expansion, and providing immune surveillance of B-cells.
• CD70 ligation activates the TRAF2-PTPN6 axis, which inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory.
• As a member of the tumor necrosis factor receptor superfamily (TNFRSF7), CD27 contains characteristic cysteine-rich repeats and is a transmembrane glycoprotein with disulfide bonds.
• CD27 localizes to the cell membrane and includes a signal peptide and a single transmembrane helix.
• In hematopoietic regulation, CD70-CD27 signaling on early progenitor cells provides a negative feedback signal to modulate leukocyte differentiation during immune activation.
• In adipose tissue, CD27 negatively regulates the function of Th2 lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 20-180 of pig CD27, suggesting recognition of the extracellular domain. Validate reactivity in the relevant sample system.
• For flow cytometry (FC) applications, use standard cell surface staining protocols with appropriate isotype controls.
• Given the costimulatory role of CD27 in T-cell activation, consider using this antibody to monitor CD27 expression in T-cell stimulation assays.
• Ensure optimal antibody titration to achieve the best signal-to-noise ratio in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CD27-related research and immunoassay development, we offer a range of validated antibody pairs, monoclonal antibodies, and ancillary reagents to support your projects. Our sourcing network can accommodate rare targets and bulk requirements, ensuring reliable supply for your diagnostic needs.