Anti-PIF4 Rabbit Polyclonal Antibody for WB and ELISA - Q8W2F3

Core Product Specifications and Parameters

Biological Background: Transcription Factor PIF4 Function and Localization

• Transcription factor PIF4, also known as AtPIF4, bHLH9, EN102, and SRL2, is a basic helix-loop-helix protein encoded by the PIF4 gene in Arabidopsis thaliana. It integrates light and temperature signals to regulate growth.
• Functions negatively in phytochrome B signaling, controlling expression of genes involved in cell expansion by binding to G-box motifs (By similarity).
• Activated by cryptochromes CRY1 and CRY2 under low blue light, directly binding to E-box variants (5'-CA[CT]GTG-3') in chromatin to stimulate gene expression for adaptive responses such as hypocotyl elongation. Related references: PMID:26724867
• Cooperates with transcriptional coactivator PTAC12/HMR/PAP5 and mediator subunit MED14 to regulate thermoresponsive growth genes, mainly those involved in auxin biosynthesis and signaling, promoting thermomorphogenesis like hypocotyl elongation in warm temperatures. Related references: PMID:30635559 PMID:33824329 PMID:36063057
• Localizes exclusively to the nucleus, consistent with its role as a transcription factor.
• Predominantly expressed in leaves, stems, and seedlings, with lower levels in fruits, flowers, and roots, indicating its importance in vegetative growth.
• Subject to alternative splicing, generating protein variants that may fine-tune its activity across different developmental and environmental contexts.
• Molecular weight: 48.4 kDa (calculated from sequence); annotated as a reference proteome and involved in phytochrome signaling, transcription regulation, and stress responses.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 300-400 of Arabidopsis PIF4 (NP_565991.2). Given the polyclonal nature of this antibody, it may recognize multiple epitopes and potentially cross-react with closely related bHLH proteins; validation in the specific experimental system is recommended.
• For Western blot, use whole-cell or nuclear-enriched extracts from Arabidopsis thaliana tissues such as seedlings or leaves, where PIF4 is highly expressed. Optimal detection may require optimization of blocking and antibody dilution.
• This antibody is suitable for ELISA, which can be employed for quantitative detection of PIF4 protein levels in plant lysates. Consider using a standard curve with recombinant PIF4 protein for accurate quantification.
• Since PIF4 functions in light and temperature signaling pathways, experimental conditions (light regime, temperature) should be carefully controlled to avoid unintended modulation of PIF4 expression.
• The predicted molecular weight is 48 kDa; monitor for potential degradation bands, and include protease inhibitors during sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive one-stop platform for IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. This PIF4 rabbit polyclonal antibody is part of our extensive portfolio for plant biology and stress response research, complementing our offerings of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material sourcing. Let CamelBio be your trusted partner in accelerating discovery and assay development.