Anti-PIEZO1/FAM38A Polyclonal Antibody for WB, ELISA - Q92508

Core Product Specifications and Parameters

Biological Background: PIEZO1 Function and Localization

• PIEZO1 (also known as FAM38A and Mib) is a pore-forming subunit of the mechanosensitive non-specific cation Piezo channel, essential for rapidly adapting mechanically activated currents and touch sensation. Related references: PMID:23479567, PMID:23695678, PMID:25955826
• Piezo channels form homotrimeric three-blade propeller-shaped structures and utilize a cap-motion and plug-and-latch mechanism to gate ion-conducting pathways. Related references: PMID:37590348
• The channel conducts monovalent alkali ions most efficiently for K+, and also permeates divalent ions except Mn2+, which reduce K+ currents. Related references: PMID:25955826
• Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER and stimulation of calpain signaling. Related references: PMID:20016066
• Acts as a shear-stress sensor in endothelial cells, promoting cell organization and alignment in the direction of blood flow via calpain activation. Related references: PMID:25119035
• Functions as a sensor of phosphatidylserine flipping at the plasma membrane, governing myotube formation through Ca2+ influx and Rho GTPase signaling. Related references: PMID:29799007
• Subcellularly localized to the endoplasmic reticulum membrane, ER-Golgi intermediate compartment membrane, and cell membrane, including lamellipodia.
• Tissue expression includes neurons, erythrocytes, myoblasts, and activated astrocytes in Alzheimer disease brains; post-translational modifications include glycosylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 1811–1952) resides in the C‑terminal region of PIEZO1; consider its accessibility and topology in your assay design.
• For Western blot, expect a band at ~287 kDa; use low‑percentage acrylamide gels and optimized transfer conditions for high‑molecular‑weight proteins.
• This polyclonal antibody is qualified for ELISA; performance may vary with coating antigen and detection format—validate in your specific system.
• Cross‑reactivity is confirmed only for human; for other species, verify reactivity experimentally before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one‑stop access to validated IVD raw materials, including this PIEZO1/FAM38A polyclonal antibody for mechanobiology and vascular studies. Beyond single reagents, we supply matched antibody pairs, optimized monoclonals, ancillary bulk reagents, and can source rare‑target raw materials to support your complete workflow from discovery to clinical application.