Anti-PIEZO1 (FAM38A) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92508

Core Product Specifications and Parameters

Biological Background: PIEZO1 (FAM38A) Function and Localization

• PIEZO1 (Protein FAM38A, Mib) is a pore-forming subunit of the mechanosensitive non-specific cation channel essential for sensing touch and tactile pain.
  Related references:
  PMID:23479567 PMID:23695678 PMID:25955826
• The channel assembles as a homotrimeric three-blade propeller, using a cap-motion and plug-and-latch gating mechanism to control ion influx.
  Related references:
  PMID:37590348
• Conductance is highest for K+, intermediate for Na+ and lowest for Li+; divalent ions permeate slowly and can suppress K+ fluxes. The channel is inhibited by ruthenium red and gadolinium.
  Related references:
  PMID:25955826
• Primary localization includes the endoplasmic reticulum (ER) membrane, ER–Golgi intermediate compartment, and plasma membrane; it is also detected in lamellipodium membrane.
• Plays a key role in epithelial cell adhesion by maintaining integrin activation through R‑Ras recruitment and calpain signaling at the ER.
  Related references:
  PMID:20016066
• Acts as a shear‑stress sensor in endothelial cells, promoting cell alignment along blood flow via calpain activation.
  Related references:
  PMID:25119035
• In myoblasts, senses phosphatidylserine flipping and triggers Ca2+-dependent Rho GTPase signaling, leading to actomyosin assembly and myotube formation.
  Related references:
  PMID:29799007
• Tissue expression: widespread, including neurons, erythrocytes, and myoblasts; in Alzheimer disease, expressed in activated astrocytes around senile plaques.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2230–2420 within the C‑terminal intracellular region; consider using this antibody to probe C‑terminal epitopes or detect recombinant fragments of PIEZO1.
• For Western blotting, note that PIEZO1 has a predicted mass of ~287 kDa; use a low‑percentage SDS‑PAGE gel (e.g., 6–8%) and optimize transfer conditions (e.g., extended time, low methanol) for high‑molecular‑weight proteins.
• For immunofluorescence/ICC, observe punctate cytoplasmic staining (ER/Golgi) as well as plasma membrane and lamellipodial localization; co‑stain with organelle markers and validate with appropriate controls.
• In ELISA applications, performance may depend on the target protein conformation; consider using recombinant protein standards or known positive samples for assay calibration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw‑material procurement, technical services, and consulting—from concept to clinic. This anti‑PIEZO1 antibody supports research in mechanobiology, touch/pain sensation, vascular biology, and ion‑channel physiology. Beyond antibodies, CamelBio can supply validated antibody pairs, optimized monoclonal reagents, bulk ancillary proteins, and source difficult‑to‑find targets to accelerate your diagnostic or research development.