Anti-PIDD Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9HB75

Core Product Specifications and Parameters

Biological Background: PIDD Function and Localization

• PIDD (p53-induced death domain-containing protein 1) is also known as leucine-rich repeat and death domain-containing protein (LRDD). It plays a dual role in cell fate decisions downstream of p53, mediating either survival or apoptosis in response to DNA damage and stress. Related references: PMID:10973264 PMID:15073321 PMID:16360037
• It forms the PIDDosome complex with CRADD and CASP2, leading to CASP2 activation and the induction of apoptosis. Related references: PMID:15073321 PMID:17159900
• PIDD interacts with IKBKG and RIPK1 to promote sumoylation and ubiquitination of IKBKG, which is critical for NF-kappa-B transcription factor activation. Related references: PMID:16360037 PMID:17159900
• The protein localizes to both the cytoplasm and nucleus.
• PIDD is ubiquitously expressed across tissues.
• It undergoes acetylation and phosphorylation; contains leucine-rich repeats and a death domain; alternative splicing generates multiple isoforms; mutations are associated with intellectual disability.
• The full-length protein has a calculated molecular weight of approximately 100 kDa (99.7 kDa).

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-85), conserved across isoforms, so the antibody may detect multiple PIDD variants; validate isoform specificity in your model system.
• For Western blot, a band near 100 kDa is expected; use appropriate positive and negative controls to confirm target identity.
• The antibody is applicable in IHC-P and IF/ICC; optimize fixation, permeabilization, and antigen retrieval steps for your particular samples.
• With reported cross-reactivity to human, mouse, and rat, the antibody can be considered for cross-species studies; however, verify reactivity in your species and tissue context.
• For ELISA, consider pairing with a suitable detection reagent, and validate sensitivity and specificity in your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and research laboratories in acquiring high-quality antibodies, antigen reagents, and ancillary materials. The PIDD antibody is part of CamelBio’s portfolio targeting DNA damage and apoptosis pathways, essential for cancer and cell signaling research. Explore our comprehensive range of validated antibody pairs, recombinant proteins, and bulk reagent options to streamline your assay development and supply needs.