Anti-PID1 Polyclonal Antibody for WB, IP, ELISA - Q7Z2X4

Core Product Specifications and Parameters

Biological Background: PID1 Function and Localization

• PID1, also known as NYGGF4 or PCLI1, is a PTB domain-containing protein that interacts with cubilin and LRP1, suggesting a role in endocytic or signaling pathways.
• It promotes the proliferation of preadipocytes without affecting their differentiation, implicating it in adipose tissue expansion and obesity-related processes.
• Alternative splicing generates multiple isoforms, which may exhibit tissue-specific functions or regulatory differences.
• PID1 is predominantly localized in the cytoplasm.
• It is widely expressed across tissues, including subcutaneous fat, heart, skeletal muscle, brain, and many others, indicating broad regulatory roles.
• The protein is phosphorylated, hinting at regulation by kinase-mediated signaling cascades.
• In summary, PID1 is a multi-functional adaptor protein with emerging roles in cell proliferation and metabolic tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 124–248 of human PID1; consider verifying reactivity with your target isoform if multiple isoforms exist.
• For Western blotting, endogenous PID1 runs at approximately 28 kDa; use a positive control lysate from tissues like adipose, heart, or skeletal muscle where PID1 is expressed.
• When performing immunoprecipitation, optimize antibody concentration and incubation conditions with your specific lysate.
• ELISA applications may require coating antigen or capture antibody; validate linearity and sensitivity using recombinant PID1 protein as a standard.
• Cross-reactivity with mouse and rat is reported, but always confirm reactivity in your experimental system due to potential species-specific variations.
• Store this antibody upon arrival as recommended (ice bag shipment suggests maintaining cold chain); avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a single access point for high-quality IVD raw materials, technical support, and consulting—from concept to clinical application. For adipocyte biology and metabolic disease research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-PID1 pAb, bulk ancillary reagents, and can assist with rare-target raw-material sourcing to accelerate your assay development.