Anti-PICK1 Polyclonal Antibody for WB, ELISA - Q9NRD5

Core Product Specifications and Parameters

Biological Background: PICK1 Function and Localization

• PICK1 (PRKCA-binding protein) is an adapter protein that organizes subcellular localization of membrane proteins containing PDZ recognition sequences, also known as protein interacting with C kinase 1.
• It clusters various receptors and regulates their internalization, playing a key role in synaptic plasticity through AMPA receptor trafficking.
• PICK1 regulates actin polymerization by inhibiting the Arp2/3 complex, affecting neuronal morphology, AMPAR endocytosis, and spine shrinkage during long-term depression.
• In astrocytes, PICK1 antagonistically regulates morphology versus the WASL/N-WASP Arp2/3 complex activator.
• It may modulate ASIC1/ASIC3 channel activity and is potentially regulated by PRKCA activation.
• Ubiquitously expressed, PICK1 localizes to the cytoplasm, perinuclear region, membrane, postsynaptic density, synapse, synaptosome, and cytoskeleton.
• The protein possesses actin-binding, metal-binding (zinc, calcium), lipoprotein, palmitate, phosphoprotein, and alternative splicing features, with a UniProt molecular weight of 46600 Da.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 300-400 of human PICK1; ensure the target epitope is accessible in native protein detection.
• For Western blot, consider testing in human-derived cell lysates or tissue extracts; optimize antibody dilution to detect the ~47 kDa band.
• ELISA applications may benefit from using recombinant PICK1 protein as a positive control to establish assay sensitivity.
• Cross-reactivity is reported for human; if using other species, validate reactivity independently due to potential sequence variations.
• This polyclonal antibody may exhibit higher sensitivity in capturing multiple epitopes but requires careful lot-to-lot consistency assessment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a reliable one-stop portal for IVD raw materials, including validated anti-PICK1 polyclonal antibodies like this product. With expertise in neuroscience and signaling research, we provide bulk ancillary reagents, optimized antibody pairs, and rare target sourcing from concept to clinic. Let us support your next assay development with responsive, B2B-tailored supply solutions.