Anti-PIBF1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8WXW3

Core Product Specifications and Parameters

Biological Background: PIBF1 Function and Localization

• PIBF1 (Progesterone-induced-blocking factor 1), also known as CEP90, JBTS33, or PIBF, is a multi-functional protein with both intracellular centrosomal and secreted immunomodulatory roles.
• The centrosomal form is a pericentriolar protein essential for mitotic spindle pole integrity, centrosomal accumulation of PCM1, recruitment of centriolar satellite proteins like BBS4, and primary cilia formation. Related references: PMID:21224392 PMID:23110211
• It cooperates with CEP63 to promote centriole duplication and centrosomal localization of CDK2. Related references: PMID:26297806
• The secreted isoform mediates progesterone effects by modulating phospholipase A2 activity and arachidonic acid metabolism, inducing a Th2-biased immune response and inhibiting NK cell activity to exert an anti-abortive effect. Related references: PMID:12516630 PMID:14634107 PMID:3863495
• Secreted PIBF1 activates STAT6 and inhibits STAT4 phosphorylation through JAK/STAT signaling, potentially involving IL4R and a GPI-anchored protein. Related references: PMID:16393965 PMID:25218441
• Subcellular localization includes the centrosome, centriolar satellites, cytoplasm, nucleus, and secretion, reflecting its diverse functions.
• Tissue expression is highest in testis, with moderate levels in spleen, thymus, prostate, ovary, small intestine, and colon; it is also expressed in first-trimester pregnancy decidua and trophoblasts, and overexpressed in various solid tumors and leukemias.
• Keywords: Alternative splicing, Ciliopathy, Disease variant, Immunity, Joubert syndrome, Secreted. UniProt: PIBF1_HUMAN, MW ~90 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human PIBF1, raising an antibody that may recognize both full-length and truncated isoforms. Validate endogenous detection in the relevant sample system.
• For Western blot, the protein weight is approximately 90 kDa; multiple bands may appear due to alternative splicing or post-translational modifications. Consider using knockdown or knockout controls to confirm specificity.
• In IHC-P, anticipate staining in tissues with known PIBF1 expression such as testis, placenta, or tumor samples. Optimize antigen retrieval and antibody concentration for your specific tissue and protocol.
• For ELISA, the polyclonal antibody can be used as capture or detection reagent; test against recombinant or native protein to determine binding efficiency.
• Cross-reactivity is reported for human, mouse, and rat; verify with positive and negative controls for each species in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers IVD manufacturers and researchers a comprehensive portfolio of raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. This PIBF1 antibody supports studies in ciliogenesis, reproductive immunology, and cancer, and can be sourced along with matched detection pairs or custom formulations. With end-to-end support from concept to clinic, CamelBio simplifies procurement for reproducible and scalable diagnostic development.