Anti-PIBF1 Rabbit Polyclonal Antibody for WB, ELISA - Q8WXW3

Core Product Specifications and Parameters

Biological Background: PIBF1 Function and Localization

• Progesterone-induced-blocking factor 1 (PIBF1) is a multifunctional protein also known as Centrosomal protein of 90 kDa (CEP90), encoded by the PIBF1 gene with several splice isoforms.
• Acts as a pericentriolar protein required to maintain mitotic spindle pole integrity, and is essential for the centrosomal accumulation of PCM1 and recruitment of centriolar satellite proteins such as BBS4, thereby playing a role in primary cilia formation. Related references: PMID:21224392, PMID:23110211
• Required for CEP63 centrosomal localization and its interaction with WDR62; together with CEP63, promotes centriole duplication and facilitates the centrosomal enrichment of CDK2. Related references: PMID:26297806
• The secreted form mediates progesterone’s immunomodulatory effects by modulating phospholipase A2 activity and arachidonic acid metabolism, inducing a Th2-biased immune response and exerting an anti-abortive function via control of decidual natural killer (NK) cells. Related references: PMID:12516630, PMID:14634107, PMID:3863495
• Increases production of Th2-type cytokines through JAK/STAT pathway signaling, activating STAT6 and inhibiting STAT4 phosphorylation; the signaling involves an unidentified receptor that appears to implicate IL4R and a GPI-anchored protein. Related references: PMID:16393965, PMID:25218441
• Localizes primarily to the centrosome and centriolar satellites, but is also found in the cytoplasm, nucleus, and can be secreted; its dynamic subcellular distribution underpins both its mitotic and immunoregulatory roles.
• Expressed at highest levels in testis, with moderate expression in spleen, thymus, prostate, ovary, small intestine, and colon; also detected in first trimester pregnancy decidua and trophoblast populations, and overexpressed in various solid tumors and hematopoietic cancers. Related references: PMID:11935316, PMID:12516630, PMID:18817979, PMID:25218441
• Classified under keywords including Alternative splicing, Ciliopathy, Disease variant (Joubert syndrome), Immunity, and Secreted; highlighting its broad biological relevance and clinical significance in ciliopathies and immune disorders.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 598–757, a region within the C-terminal portion of PIBF1; for Western blot, a band migrating around 90 kDa is expected. Validate detection in a relevant positive control lysate, such as testis or placenta tissue, or cell lines known to express PIBF1.
• For ELISA applications, ensure optimal antigen coating using the immunogen sequence or full-length protein; due to the polyclonal nature, consider potential lot-to-lot variability and include appropriate controls to confirm specificity.
• Given that PIBF1 has both centrosomal and secreted forms, consider the biological context of your samples (e.g., cell fractionation for centrosome enrichment vs. culture supernatant for secreted form) when planning experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to provide one-stop access to high-quality IVD raw materials, including validated antibodies like this anti-PIBF1 polyclonal antibody for centrosome biology and immunoregulation studies. Beyond this product, we offer optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and dedicated sourcing for rare and challenging targets, covering every stage from concept to clinic. Let CamelBio be your reliable sourcing bridge for advancing diagnostic development and life science research.