Anti-PIAS4 Polyclonal Antibody for WB, ELISA - Q8N2W9

Core Product Specifications and Parameters

Biological Background: PIAS4 Function and Localization

• Serves as an E3 SUMO ligase and SUMO-tethering factor, stabilizing UBE2I–substrate interactions to catalyze SUMO conjugation. Related references: PMID:12511558 PMID:12631292 PMID:12727872
• Sumoylates a broad range of substrates including ALKBH5, AXIN1, CEBPA, KLF8, GATA2, PARK7, HERC2, MYB, TCF4, and RNF168, modulating their activities. Related references: PMID:12223491 PMID:12511558 PMID:12631292
• Operates as a transcriptional coregulator in STAT, p53/TP53, Wnt, and steroid hormone signaling pathways. Related references: PMID:11388671
• Participates in gene silencing processes. Related references: PMID:11248056
• In the Wnt signaling cascade, represses LEF1 and enhances TCF4 transcriptional activity via their SUMO modification. Related references: PMID:12727872 PMID:15831457
• Catalyzes SUMO2 conjugation to KAT5 in response to DNA damage, facilitating homologous recombination repair of double-strand breaks. Related references: PMID:32832608
• Mediates PARP1 sumoylation upon PARP1 trapping to chromatin. Related references: PMID:35013556
• Localizes to the nucleus and PML nuclear bodies; binds AT-rich matrix/scaffold attachment regions (MARs/SARs).
• Highly expressed in testis, with lower levels in spleen, prostate, ovary, colon, and peripheral blood leukocytes.
• Contains a zinc-finger domain and is subject to acetylation; molecular weight approximately 56.5 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 60–260, a region predicted to be surface-exposed in the native protein; this may contribute to recognition of both native and denatured PIAS4.
• For WB, consider probing whole-cell lysates from human or mouse cell lines (e.g., HeLa, HEK293, or mouse testis extracts) and expect a band near 57 kDa; verify specificity using knockdown or knockout controls.
• For ELISA, use recombinant PIAS4 protein or peptide encompassing the immunogen sequence as coating antigen; optimize coating concentration and antibody dilution in your assay buffer.
• Since this is a polyclonal antibody, lot-to-lot consistency should be confirmed by titration and comparison against a reference standard if used in quantitative assays.
• Cross-reactivity with mouse PIAS4 is reported, but homologous proteins in other species should be evaluated by sequence alignment before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This PIAS4 antibody is part of our extensive portfolio for SUMOylation and transcriptional regulation research; we also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your IVD development with reliable, high-quality reagents.