Anti-PIAS3 Rabbit Polyclonal Antibody for IF/ICC and ELISA - Q9Y6X2

Core Product Specifications and Parameters

Biological Background: PIAS3 Function and Localization

• Serves as an E3 SUMO ligase and SUMO-tethering factor, stabilizing the interaction between UBE2I and substrate proteins.
• Acts as a transcriptional coregulator in STAT and steroid hormone signaling pathways; inhibits STAT3 DNA-binding activity, leading to suppressed cell growth.
• Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Related references: PMID:21965678 PMID:9388184
• Sumoylates CCAR2, which promotes its interaction with SIRT1. Related references: PMID:25406032
• Diminishes sumoylation of ZFHX3 by preventing colocalization of ZFHX3 with SUMO1 in the nucleus. Related references: PMID:24651376
• Localizes to the cytoplasm, nucleus, and nuclear speckles.
• Contains a zinc-finger domain and binds zinc, characteristic of the PIAS protein family.
• Expressed widely across human tissues.

Experimental Guidance and Technical Tips

• For IF/ICC, consider testing various fixation and permeabilization conditions to detect PIAS3 in both cytoplasmic and nuclear compartments.
• In ELISA, the antibody may be used with recombinant PIAS3 or endogenous protein in cell lysates; validate assay conditions for optimal signal.
• As a polyclonal antibody, verify lot-to-lot consistency for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets. This anti-PIAS3 antibody supports investigations into STAT signaling and SUMO-mediated pathways, relevant to oncology and cell growth research. For large-scale procurement or tailored solutions, CamelBio offers end-to-end support from concept to clinical application.