Anti-PIAS2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75928

Core Product Specifications and Parameters

Biological Background: PIAS2 Function and Localization

• PIAS2 (Protein Inhibitor of Activated STAT2) is an E3 SUMO-protein ligase that facilitates SUMO conjugation to target proteins, acting as both a SUMO ligase and a SUMO-tethering factor.
• It functions as a transcriptional coregulator in key signaling pathways, including the STAT, p53, and steroid hormone pathways, primarily mediating gene silencing depending on the biological context.
• Alternative splicing generates distinct isoforms (e.g., PIAS2-alpha and PIAS2-beta) with different substrate specificities; for example, PIAS2-beta preferentially sumoylates MDM2, while PIAS2-alpha targets PARK7 and PML.
• The protein localizes to nuclear speckles, PML bodies, and the nucleoplasm, consistent with its role in nuclear signaling and transcription regulation.
• PIAS2 is predominantly expressed in the testis, with isoform 3 being the major form in adult testis and present at lower levels in the pancreas and other tissues.
• It contains zinc-finger domains and is classified under metal-binding proteins, phosphoproteins, and proteins involved in the ubiquitin-like conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 420–621 of human PIAS2 (isoform alpha), which may allow the antibody to recognize both alpha and beta isoforms; however, isoform-specific detection should be confirmed experimentally.
• For western blotting, an expected band around 68 kDa should be observed; consider using testis lysates or PIAS2-overexpressing cell lines as positive controls.
• In IHC-P applications, antigen retrieval conditions may need optimization; testis sections are recommended as positive tissue due to high PIAS2 expression.
• Pre-absorption with the immunogen or siRNA knockdown can be used to confirm specificity; cross-reactivity with mouse enables studies in rodent models but requires validation in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop sourcing of IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. This anti-PIAS2 antibody is suitable for SUMOylation and transcription regulation research, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Our supply capabilities help streamline your raw material procurement, whether for routine assays or specialized projects.