Anti-PI4K2B Rabbit Polyclonal Antibody for WB, ELISA - Q8TCG2

Core Product Specifications and Parameters

Biological Background: PI4K2B Function and Localization

• PI4K2B, also known as phosphatidylinositol 4-kinase type II-beta, is a lipid kinase that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P). Related references: PMID:11923287, PMID:12324459
• This reaction is the first committed step in the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messengers inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol. Related references: PMID:11923287, PMID:12324459
• Together with PI4K2A and type III PI4Ks, PI4K2B contributes to cellular PI4-kinase activity, particularly significant at the plasma membrane, endosomal, and Golgi compartments. Related references: PMID:11923287, PMID:12324459
• PI4K2B is involved in the production of InsP3 in stimulated cells and likely regulates vesicular trafficking, linking phosphoinositide signaling to membrane dynamics.
• The protein localizes to multiple membrane compartments, including the Golgi apparatus membrane, endoplasmic reticulum membrane, cell membrane, and early endosome membrane, as well as the cytosol.
• PI4K2B is widely expressed in human tissues, underscoring its fundamental role in cellular phosphoinositide metabolism.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein encompassing amino acids 1–120 of human PI4K2B (NP_060793.2). This N-terminal region may be accessible for detection; consider validating epitope specificity in your experimental system.
• For Western blot (WB), a predominant band around 55 kDa is expected based on the theoretical molecular weight; post-translational modifications or isoform variants might result in additional bands.
• ELISA is a suggested application; optimize antibody dilution and coating conditions for your assay format.
• The antibody is reactive with human PI4K2B; cross-reactivity with other species or PI4K family members (e.g., PI4K2A) has not been reported. Assess specificity if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated polyclonal antibodies like this anti-PI4K2B antibody. Our offerings encompass premium antibody pairs, bulk reagents, and rare-target raw-material sourcing, supporting your work on lipid kinase signaling and phosphoinositide metabolism from concept to clinic.