Anti-PI3K p85α/p55γ/p85β Polyclonal Antibody for WB, IF/ICC, ELISA - P27986 / O00459 / Q92569

Core Product Specifications and Parameters

Biological Background: PI3K p85α/p55γ/p85β (PIK3R1/PIK3R2/PIK3R3) Function and Localization

• PI3K regulatory subunits p85α (PIK3R1), p55γ (PIK3R3), and p85β (PIK3R2) are non-catalytic adapters that link the p110 catalytic subunit to activated receptor tyrosine kinases. p85α contains two SH2 domains and an SH3 domain, binding phosphorylated tyrosine residues on receptors such as FGFR, KIT, PDGFR, and integrin β2 (ITGB2), thereby mediating PI3K membrane recruitment and activation. Related references: PMID:17626883 PMID:19805105 PMID:7518429
• The p85α subunit plays an essential role in insulin signaling, promoting glucose uptake and glycogen synthesis in insulin-sensitive tissues, and modulates the cellular response to ER stress by facilitating XBP1 isoform 2 nuclear translocation during metabolic overloading in the liver. Related references: PMID:20348923
• These regulatory subunits exist as multiple isoforms: PIK3R1 produces isoforms p85α, p55α, and p50α via alternative splicing, while PIK3R2 encodes p85β and PIK3R3 encodes p55γ. Isoform 2 (p50α) of PIK3R1 is predominantly expressed in skeletal muscle and brain, with lower levels in kidney and cardiac muscle.
• Subcellularly, the p85α protein localizes primarily to the cytoplasm, where it interacts with both receptor complexes and the p110 catalytic subunit.
• Post-translational modifications, including acetylation, phosphorylation, and ubiquitination, regulate p85α stability and signaling; the protein is also a known host factor for viral interactions (KEYWORDS: Acetylation, Phosphoprotein, Ubl conjugation, Host-virus interaction).
• The predicted molecular weights reflect the isoform diversity: p85α and p85β migrate at approximately 81–84 kDa, whereas the shorter p55γ and p50α isoforms are detected around 42–54 kDa.

Experimental Guidance and Technical Tips

• The immunogen was designed using a conserved peptide sequence within amino acids 400–500, shared among PIK3R1, PIK3R2, and PIK3R3, enabling recognition of multiple regulatory subunit isoforms and cross-reactivity with human, mouse, and rat samples.
• For Western blotting (WB), validate the primary antibody dilution in your specific sample system; a starting range of 1:500–1:2000 is often used for rabbit polyclonals, but optimization is critical to resolve the expected bands at ~42–54 kDa (p55γ/p50α) and ~81–84 kDa (p85α/p85β).
• In immunofluorescence/immunocytochemistry (IF/ICC), include appropriate controls (e.g., non-immunized rabbit IgG) and consider permeabilization protocols that preserve cytoplasmic epitopes.
• For ELISA, the antibody may be used as a capture or detection reagent; pair it with a suitable p110 catalytic subunit antibody to set up sandwich formats for studying PI3K interaction dynamics.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research labs with streamlined access to a broad portfolio of validated antibodies and ancillary reagents. This anti-PI3K regulatory subunit polyclonal antibody targets key nodes in insulin and growth factor signaling, making it a valuable tool for diagnostic development in metabolic disease, oncology, and cell signaling research. Beyond this product, CamelBio offers custom bulk production, optimized antibody pairs, and sourcing of rare-target raw materials to support your entire development workflow—from early feasibility to clinical application.