Anti-PI3K-gamma Rabbit Monoclonal Antibody for WB and ELISA - P48736

Core Product Specifications and Parameters

Biological Background: PI3K-gamma Function and Localization

• PI3K-gamma (p110γ) is the catalytic subunit of class IB phosphoinositide 3-kinase, which phosphorylates PIP2 to generate the second messenger PIP3 at the plasma membrane, initiating downstream signaling networks.
• PIP3 recruits PH domain-containing proteins such as AKT1 and PDK1 to the membrane, activating pathways that regulate cell growth, survival, proliferation, and metabolism.
• Through GPCR activation, PI3K-gamma is a central mediator of immune and inflammatory responses, orchestrating leukocyte chemotaxis, integrin-mediated adhesion, actin remodeling, and respiratory burst in neutrophils.
• Together with PIK3CD (p110δ), it plays critical roles in the development, migration, and effector functions of T lymphocytes, B lymphocytes, natural killer (NK) cells, and dendritic cells.
• Exhibits both lipid kinase and serine/threonine protein kinase activities; its scaffolding functions contribute to cardiac contractility regulation by modulating cAMP levels and facilitating beta-adrenergic receptor internalization.
• Primarily localizes to the cytoplasm and cell membrane, and is expressed in tissues such as pancreas, skeletal muscle, liver, and heart.
• Classified as a phosphoprotein and implicated in angiogenesis, endocytosis, and inflammatory response pathways.

Experimental Guidance and Technical Tips

• For Western blot detection, an 8–10% resolving gel is recommended given the 126 kDa molecular weight; use extended transfer times and low-percentage methanol to ensure efficient blotting of high‑molecular‑weight proteins.
• Validate reactivity in cell lines with reported PI3K-gamma expression—for example, RAW 264.7 (mouse macrophage), Jurkat (human T‑cell), or isolated human PBMCs—and verify specificity using siRNA knockdown or CRISPR‑edited controls.
• In ELISA, consider employing the recombinant immunogen (amino acids 130–342) as a standard or coating control, and optimize blocking agents to reduce background from complex sample matrices.
• Since the antibody recognizes both human and mouse PI3K-gamma, it can facilitate cross‑species comparisons; always confirm consistent performance in your specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a one‑stop supply of IVD raw materials, including validated antibodies and recombinant proteins tailored for immunoassay development. This anti‑PI3K‑gamma monoclonal antibody supports immunological and cardiovascular research, and we offer bulk quantities, customized formulations, and assistance in sourcing rare‑target reagents. Partner with CamelBio to streamline your project from concept to clinical application.