Anti-PI3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P19957

Core Product Specifications and Parameters

Biological Background: PI3 Function and Localization

• PI3, also referred to as Elafin, SKALP, ESI, or cementoin, is a 12 kDa secreted serine protease inhibitor of the whey acidic protein (WAP) family.
• It functions primarily as an inhibitor of neutrophil and pancreatic elastase, thereby protecting tissues, especially the skin, from excessive proteolytic damage.
• PI3 has been shown to inhibit the alpha-4-beta-2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1 (hERG) and TRPV1 ion channels (PubMed:29483648). Related references: PMID:29483648
• The protein is secreted extracellularly, consistent with its role in modulating protease activity in the extracellular matrix and innate immune defense.
• Structurally, PI3 contains four disulfide bonds and a characteristic WAP domain, which is essential for its protease inhibitory function.
• It is also known as skin-derived antileukoproteinase (SKALP), reflecting its abundant expression in human epidermis and its involvement in inflammatory skin diseases.
• Encoded by the PI3 gene, it belongs to the WFDC (WAP four-disulfide core) gene cluster, whose members play roles in tissue protection and antimicrobial defense.

Experimental Guidance and Technical Tips

• Given the low molecular weight of PI3 (12 kDa), ensure the use of appropriate membrane pore sizes (e.g., 0.2 µm) and optimized transfer conditions for Western blotting to prevent protein loss.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods suitable for small secreted proteins, and validate the staining specificity in relevant positive and negative tissue controls.
• The immunogen (aa 23-117) corresponds to the mature protein after signal peptide cleavage, indicating that the antibody detects the secreted form; this should be considered when analyzing cellular versus extracellular localization.
• In ELISA applications, pairing with a high-affinity monoclonal detection antibody may enhance sensitivity and dynamic range for quantifying PI3 levels in complex biological samples.
• Test the antibody in the relevant species (human, mouse) under your own experimental conditions, as cross-reactivity and optimal working dilutions must be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, supporting diagnostic manufacturers and research laboratories with a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. With expertise in rare target sourcing and bulk supply, we streamline the transition from concept to clinic. This PI3 polyclonal antibody exemplifies our commitment to offering high-quality reagents for elastase and skin inflammation research, backed by reliable supply and technical support.