Anti-PI3 Kinase p85 beta Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00459

Core Product Specifications and Parameters

Biological Background: PI3 Kinase p85 beta Function and Localization

• Encoded by the PIK3R2 gene, PI3 Kinase p85 beta (phosphatidylinositol 3-kinase regulatory subunit beta) is an 85 kDa regulatory subunit of class IA PI3K.
• It associates with the p110 catalytic subunit to form active PI3K, which phosphorylates PtdIns(4,5)P2 to produce the second messenger PIP3 at the plasma membrane.
• PIP3 recruits PH domain-containing proteins such as AKT1 and PDPK1, activating signaling cascades that drive cell growth, survival, proliferation, motility, and morphology.
• Through its SH2 domain, p85 beta binds to phosphorylated protein-tyrosine kinases (e.g., activated growth factor receptors), functioning as an adapter to localize the p110 catalytic subunit to the membrane.
• It has been shown to indirectly regulate autophagy (PubMed:23604317). Related references: PMID:23604317
• p85 beta promotes nuclear translocation of XBP1 isoform 2 under ER stress and/or insulin stimulation in the liver, thereby contributing to improved glucose tolerance.
• The protein contains SH2 and SH3 domains, is phosphorylated upon PI3K activation, and may undergo ubiquitin conjugation; mutations are associated with disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–85) of human p85 beta; this region may be less conserved, potentially providing isoform specificity, but cross-reactivity with p85 alpha should be considered and validated in the relevant sample system.
• For WB, use whole-cell lysates from human, mouse, or rat cell lines or tissues; the expected band is approximately 82 kDa. A positive control (e.g., HeLa or NIH/3T3 lysate) is recommended.
• IHC-P and IF/ICC may require optimization of antigen retrieval (heat-mediated citrate buffer, pH 6.0) and permeabilization (0.1% Triton X-100). Validate signal specificity using blocking peptide or knockout controls.
• In ELISA, this antibody can be used as a capture or detection reagent; pairing with a suitable p85 beta detection antibody is necessary for quantitative assays.
• Since p85 beta is a ubiquitously expressed intracellular protein, ensure proper fixation and permeabilization for IF/ICC, and consider co-staining with compartment-specific markers to confirm localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a one-stop supply chain for IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-PI3 Kinase p85 beta antibody, targeting a key node in PI3K/AKT signaling, is available through our platform alongside complementary raw materials for cell growth and cancer biomarker research. Leverage CamelBio’s sourcing expertise to streamline procurement of niche targets and accelerate your assay development from concept to clinic.