Anti-PI3 Kinase p85 beta Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O00459

Core Product Specifications and Parameters

Biological Background: PI3 Kinase p85 beta Function and Localization

• Encodes the phosphatidylinositol 3-kinase regulatory subunit beta (p85-beta), a key regulatory subunit of class IA PI3K.
• Forms a heterodimer with catalytic p110 subunits, phosphorylating PtdIns(4,5)P2 to generate the second messenger PIP3.
• PIP3 recruits pleckstrin homology (PH) domain-containing proteins such as AKT1 and PDPK1 to the plasma membrane, activating pathways regulating cell growth, survival, proliferation, and motility.
• Binds to activated receptor tyrosine kinases via its SH2 domain, functioning as an adapter to localize the catalytic subunit to the membrane.
• Indirectly regulates autophagy, as shown in functional studies. Related references: PMID:23604317
• Promotes nuclear translocation of XBP1 isoform 2 under ER stress or insulin stimulation in the liver, contributing to improved glucose tolerance (By similarity).
• Contains SH2 and SH3 domains and is subject to phosphorylation and ubiquitination; variants are associated with MPPH syndrome (PIK3R2-related).
• Theoretical molecular weight approximately 81.5 kDa (UniProt), migrating at ~82 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 108–298 of human PI3 Kinase p85 beta (NP_005018.1); for alternative antibodies, consider targeting different regions or orthologs.
• This polyclonal antibody is suitable for Western blot (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA. Optimize dilutions for each application using appropriate positive and negative controls.
• Given cross-reactivity with mouse and rat, the antibody may detect orthologs; validation in the specific sample system is recommended.
• For storage, aliquot and keep at -20°C or below; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, from optimized polyclonal antibodies like this anti-PI3 Kinase p85 beta product to rare target reagents. Our supply solutions include validated antibody pairs, bulk ancillary reagents, and custom sourcing for PI3K pathway studies. We support your IVD development from concept to clinic with reliable, one-stop procurement.