Anti-PI3 Kinase p85 alpha/beta Rabbit Monoclonal Antibody for WB, ELISA - P27986 / O00459

Core Product Specifications and Parameters

Biological Background: PI3 Kinase p85 alpha/beta Function and Localization

• Encoded by PIK3R1 (p85-alpha) and PIK3R2 (p85-beta), these regulatory subunits of phosphatidylinositol 3-kinase function as adaptor proteins linking the p110 catalytic subunit to activated receptor tyrosine kinases via SH2 domains.
• Binds activated protein-tyrosine kinases through its SH2 domain, mediating the membrane translocation of the p110 catalytic unit. Essential for insulin-stimulated glucose uptake and glycogen synthesis in insulin-sensitive tissues, and participates in signaling pathways downstream of FGFR, KIT, and PDGFR. Modulates ER stress responses by promoting XBP1 nuclear translocation in the liver, contributing to improved glucose tolerance. Related references: PMID:17626883 PMID:19805105 PMID:7518429 PMID:20348923
• Alternative splicing generates multiple isoforms; isoform 2 is predominantly expressed in skeletal muscle and brain, with lower levels in kidney and cardiac muscle.
• Subcellular localization is primarily cytoplasmic, where it facilitates signal transduction from membrane receptors to intracellular effectors.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, influencing its stability and activity. Keywords such as SH2 domain, SH3 domain, host-virus interaction, and disease variant (dwarfism) highlight its multifunctional roles.
• Acts as a host factor in viral interactions and is linked to developmental disorders such as SHORT syndrome (dwarfism).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 400–500 of human p85 alpha/beta, a region conserved in both isoforms, supporting recognition of alpha and beta variants.
• For Western blot (WB), validate the antibody in lysates from human or mouse cell lines known to express PI3K p85 (e.g., HeLa, 3T3-L1 adipocytes). Expected bands: ~42–54 kDa (predominantly p85-alpha) and ~81–84 kDa (p85-beta). Additional bands from splice isoforms or degradation products may appear; optimize lysate preparation and sample loading accordingly.
• For ELISA, consider pairing with a validated capture/detection antibody after in-house testing. Check cross-reactivity with related isoforms and use appropriate blocking and washing conditions to minimize background.
• Since cross-reactivity is listed for human and mouse, target sequence conservation should be verified if extending to other species; perform sequence alignment prior to application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This PI3 Kinase p85 alpha/beta monoclonal antibody is an excellent tool for studying the PI3K/AKT signaling pathway, with applications in oncology, metabolic disorders, and immunology. Beyond this product, CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare-target raw materials to support your entire IVD development pipeline.