Anti-PI3 Kinase p85 alpha Rabbit mAb for WB, IF, IHC, ELISA - P27986

Core Product Specifications and Parameters

Biological Background: PI3 Kinase p85 alpha Function and Localization

• The PIK3R1 gene encodes the p85-alpha regulatory subunit of phosphoinositide 3-kinase (PI3K), which forms a heterodimer with a catalytic p110 subunit; alternative splicing produces multiple isoforms (e.g., isoform 2 expressed in skeletal muscle and brain).
• p85-alpha acts as an adapter that binds, via its SH2 domains, to phosphorylated tyrosine residues on activated receptor tyrosine kinases (e.g., FGFR, KIT, PDGFR) and adaptor proteins, recruiting the p110 catalytic subunit to the plasma membrane for localized PIP3 production. Related references: PMID:7518429
• In insulin-responsive tissues, p85-alpha is necessary for insulin-stimulated glucose uptake and glycogen synthesis, playing a critical role in metabolic regulation.
• The protein mediates signaling downstream of multiple growth factor receptors, including FGFR1–4, KIT, PDGFRA/B, and integrin ITGB2, influencing cell growth, survival, and migration. Related references: PMID:17626883, PMID:19805105, PMID:7518429
• Subcellular localization is predominantly cytoplasmic, with stimulus-dependent translocation to the plasma membrane.
• The protein undergoes extensive post-translational modifications—phosphorylation, acetylation, and ubiquitination—that fine-tune its activity and stability.
• During endoplasmic reticulum (ER) stress, p85-alpha promotes nuclear translocation of XBP1 isoform 2 in a manner dependent on insulin or ER stress, contributing to improved glucose tolerance under metabolic overload. Related references: PMID:20348923
• Isoform 2 is enriched in skeletal muscle and brain, with lower levels in kidney and cardiac muscle; both isoform 2 and isoform 4 are detected at the protein level in skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 240–380, a region containing the inter-SH2 (iSH2) domain that mediates binding to p110 catalytic subunits; the antibody may recognize conserved epitopes across p85 isoforms.
• For Western blot, the predicted molecular weight of p85-alpha is ~84 kDa; validate with positive controls such as insulin-stimulated HepG2 or 3T3-L1 lysates, and include human, mouse, or rat samples as appropriate.
• For immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized; the antibody is suitable for both frozen (IF-P) and paraffin-embedded tissues.
• In immunofluorescence/ICC, expect cytoplasmic staining with potential membrane enrichment upon growth factor stimulation.
• ELISA applications may benefit from a matched antibody pair; cross-reactivity across human, mouse, and rat broadens utility in translational models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. For projects targeting the PI3K/AKT pathway in oncology, diabetes, or immunology, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development. Let us be your single point of contact for all raw-material needs involving PI3 Kinase p85 alpha and related signaling molecules.