Anti-PI3 Kinase p110 delta Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O00329

Core Product Specifications and Parameters

Biological Background: PI3 Kinase p110 delta Function and Localization

• Encoded by the PIK3CD gene, PI3 Kinase p110 delta is the catalytic subunit of the class I phosphoinositide-3-kinase delta isoform, which phosphorylates phosphatidylinositol and its derivatives at the 3‑position of the inositol ring to produce 3‑phosphoinositide second messengers. Related references: PMID:9235916
• Specifically, it uses ATP to convert PtdIns(4,5)P2 (phosphatidylinositol 4,5‑bisphosphate) into PIP3 (phosphatidylinositol 3,4,5‑trisphosphate), triggering the recruitment of PH domain‑containing proteins such as AKT1 and PDPK1 to the plasma membrane and activating downstream signaling cascades. Related references: PMID:15135396
• The protein is predominantly expressed in leukocytes, with the highest levels found in peripheral blood mononuclear cells, spleen, and thymus; lower expression is observed in testes, uterus, colon, and small intestine.
• Subcellularly, PI3 Kinase p110 delta localizes to the cytoplasm, where it interacts with regulatory subunits and membrane‑bound substrates.
• It plays essential roles in adaptive and innate immunity, including B‑cell development, proliferation, migration, and antibody class switching; T‑cell receptor signaling and differentiation; natural killer (NK) cell maturation and cytotoxicity; and mast cell‑mediated allergic responses.
• Two isoforms exist due to alternative splicing: isoform 1 is expressed predominantly in leukocytes, while isoform 2 shows elevated expression in some colorectal and ovarian tumors and may stabilize total RAS levels.
• The protein is subject to phosphorylation and is linked to the primary immunodeficiency disorder activated PI3K delta syndrome (APDS) when mutated.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from within amino acids 200–300 of the human protein; align the immunogen sequence with your target species to confirm cross-reactivity for non‑listed species.
• For Western blotting (WB), anticipate a band at approximately 119 kDa. Include appropriate positive and negative controls, and validate detection using a known expression system (e.g., leukocyte lysates).
• In immunohistochemistry (IHC‑P), consider antigen retrieval conditions tailored to cytoplasmic targets and optimize the primary antibody dilution using tissue known to express the protein (e.g., spleen or thymus sections).
• For ELISA assays, pair with a suitable capture/detection antibody or use the antibody as a detection reagent; validate using recombinant protein or cell lysates.
• As a rabbit monoclonal antibody, use an anti‑rabbit IgG secondary conjugate for detection in indirect formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to commercialization. For targets such as PI3 Kinase p110 delta, we offer ready‑to‑use validated antibodies—including monoclonal antibodies like this clone—along with complementary raw materials such as antibody pairs, bulk ancillary reagents, and tailored sourcing for rare or specialized targets in immunology and oncology research. Reach out to explore how our integrated supply chain can accelerate your development pipeline.