Anti-PI3 Kinase p110 beta Rabbit Monoclonal Antibody for WB and ELISA - P42338

Core Product Specifications and Parameters

Biological Background: PI3 Kinase p110 beta Function and Localization

• Protein identity: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (p110beta), encoded by the PIK3CB gene; also known as PIK3C1, P110BETA, PI3KBETA.
• Core enzymatic function: Phosphorylates PtdIns(4,5)P2 to generate PIP3, a critical second messenger that recruits AKT1 and PDK1 to the plasma membrane, activating signaling cascades for cell growth, survival, and proliferation. Related references: PMID:15135396
• Signaling downstream of GPCRs: Mediates AKT1 activation in response to ligands like CXCL12, sphingosine-1-phosphate, and lysophosphatidic acid, linking G protein-coupled receptors to PI3K/AKT signaling.
• Role in platelet function: Required for stable platelet adhesion, aggregation, and activation signaling via GPCRs, integrin αIIb/β3, and ITAM-bearing receptors; essential for thrombin and thromboxane A2-induced platelet aggregation.
• Subcellular localization: Found in both the cytoplasm and nucleus.
• Tissue specificity: Expressed ubiquitously.
• Additional functions: Involved in autophagy initiation by modulating PtdIns3P levels and PIK3C3 activity; may act as a scaffold protein independently of its lipid kinase activity. Exhibits serine/threonine protein kinase activity, including autophosphorylation. Related references: PMID:12502714
• Oncogenic signaling: The lipid kinase activity is necessary for oncogenic transformation in cells lacking PTEN and for growth of ERBB2 and RAS-driven tumors.

Experimental Guidance and Technical Tips

• For Western blot, the expected band is around 123 kDa; use appropriate molecular weight markers and validate in your sample system.
• The antibody was raised against a conserved region (aa 410–600) within the catalytic domain, which likely enables cross-reactivity with human, mouse, and rat p110β.
• For ELISA, consider using recombinant p110β protein as a standard and include appropriate positive and negative controls.
• Due to its role in PI3K complexes, native conditions may reveal interactions with regulatory subunits; denaturing conditions are recommended for Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PI3 Kinase p110 beta monoclonal antibody supports research into PI3K/AKT signaling pathways relevant to cancer, thrombosis, and cell metabolism. Beyond antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD development.