Anti-PI3 Kinase p110 alpha Rabbit Monoclonal Antibody for WB, IP, ELISA - P42336

Core Product Specifications and Parameters

Biological Background: PI3 Kinase p110 alpha Function and Localization

• PI3 Kinase p110 alpha, encoded by the PIK3CA gene, is the 110 kDa catalytic subunit of the class I phosphoinositide 3-kinase (PI3K) complex, also known as p110alpha.
• It catalyzes the phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3), a critical second messenger in numerous signaling pathways. Related references: PMID:15135396, PMID:28676499
• PIP3 recruits pleckstrin homology (PH) domain-containing proteins such as AKT1 and PDPK1 to the plasma membrane, triggering downstream cascades that regulate cell growth, survival, proliferation, and motility.
• The enzyme is activated by receptor tyrosine kinases (RTKs) in response to growth factors like EGF, insulin, IGF1, VEGFA, and PDGF, playing a key role in insulin signaling and angiogenesis.
• Beyond lipid kinase activity, PI3K p110 alpha also exhibits serine-protein kinase activity, autophosphorylating the p85 regulatory subunit and phosphorylating substrates such as 4EBP1 and H-Ras. Related references: PMID:23936502, PMID:28676499
• The protein is essential for vascular development and endothelial cell migration, particularly through VEGFA signaling and regulation of RhoA activity; it also participates in phagocytosis and pinocytosis.
• Keywords indicate it is a proto-oncogene with disease variants, and somatic mutations in PIK3CA are frequently observed in human cancers, making it a key biomarker and therapeutic target.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a specific band at approximately 124 kDa in human, mouse, and rat cell or tissue lysates. Validate protein loading and transfer efficiency.
• In immunoprecipitation (IP), validate enrichment of p110 alpha and co-immunoprecipitated proteins under native conditions, considering the antibody's reactivity with the immunogen region (aa 294–610).
• For ELISA, optimize coating conditions and antibody concentrations using recombinant p110 alpha or cell extracts; cross-reactivity with rodent species allows flexibility in model systems.
• Given the target's role in the PI3K/AKT pathway, consider using this antibody in assays studying cell proliferation, survival, or cancer biomarker analysis.
• Always include appropriate positive and negative controls to confirm specificity, especially when working with samples that may contain other PI3K isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop source for IVD raw materials. Our portfolio includes validated antibody pairs and optimized monoclonal antibodies like this anti-PI3K p110 alpha clone, suitable for developing assays targeting cancer and metabolic signaling pathways. We also offer bulk ancillary reagents and can assist in sourcing rare targets to accelerate your product development.