Anti-PHRF1 Rabbit Polyclonal Antibody for WB & ELISA - Q9P1Y6

Core Product Specifications and Parameters

Biological Background: PHRF1 Function and Localization

• PHRF1 encodes the PHD and RING finger domain-containing protein 1, also designated as RNF221 and PPP1R125.
• The gene is a member of the reference proteome, signifying its fundamental role in human biology.
• The protein possesses PHD-type and RING-type zinc finger domains, along with a coiled-coil region, indicating involvement in metal-ion coordination and potential protein-protein interactions.
• Phosphorylation sites have been identified, suggesting that its activity or localization may be regulated by kinases.
• Alternative splicing of the gene gives rise to multiple isoforms, expanding functional diversity.
• Proteomics identification has confirmed its expression in human cells.

Experimental Guidance and Technical Tips

• For Western blot, an expected band of approximately 179 kDa should be observed; consider using a high-molecular-weight marker and prolonged transfer conditions.
• In ELISA applications, the antibody can be paired with a peptide or protein coating; empirical optimization of coating antigen and antibody dilution is recommended.
• As the antibody is raised against a synthetic peptide spanning amino acids 1350–1450, verify reactivity with full-length endogenous protein in the relevant human sample system.
• When analyzing post-translational modifications like phosphorylation, ensure lysis buffers contain appropriate phosphatase inhibitors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For PHRF1-focused studies, we supply high-quality antibodies and related reagents to accelerate your development from concept to clinic. Our extensive sourcing network and technical expertise ensure reliable procurement of rare target raw materials tailored to your project needs.