Anti-PHPT1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NRX4

Core Product Specifications and Parameters

Biological Background: PHPT1 Function and Localization

• Also known as PHP, PHP14, CGI-202, HSPC141, HEL-S-132P; a 14 kDa phosphohistidine phosphatase.
• Exhibits phosphohistidine phosphatase activity, dephosphorylating phosphohistidine residues.
• Localizes to the cytoplasm.
• Expressed abundantly in heart and skeletal muscle, with lower levels in other tissues.
• Belongs to the janus family of phosphatases.
• Post-translational modifications include acetylation at specific lysine residues.
• Alternative splicing produces multiple transcript variants.
• The 3D structure has been determined, providing insights into catalytic mechanism.

Experimental Guidance and Technical Tips

• For western blotting, a band at ~14 kDa is expected; consider using heart or skeletal muscle lysate as a positive control.
• In immunohistochemistry (IHC-P), antigen retrieval may be required; validate staining in tissues with known expression patterns.
• For ELISA applications, evaluate as a capture or detection antibody in a sandwich format; pair with another clone if needed.
• The antibody reacts with human, mouse, and rat; appropriate blocking buffers and diluents should be optimized for each species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering a comprehensive portfolio including this anti-PHPT1 polyclonal antibody for phosphatase research and diagnostic assay development. Beyond this product, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to support your project from concept to clinic.