Anti-PHPT1 Rabbit Polyclonal Antibody for WB, ELISA - Q9NRX4

Core Product Specifications and Parameters

Biological Background: PHPT1 Function and Localization

• PHPT1, also known as 14 kDa phosphohistidine phosphatase, PHP, or protein janus-A homolog, is a histidine phosphatase with a molecular weight of approximately 13.8 kDa.
• The primary function of PHPT1 is to exhibit phosphohistidine phosphatase activity, removing phosphate groups from phosphohistidine residues in proteins.
• The enzyme is predominantly localized to the cytoplasm, as indicated by its SUBCELLULAR LOCATION annotation.
• PHPT1 is abundantly expressed in heart and skeletal muscle, suggesting a role in tissues with high metabolic activity.
• Post-translational modifications include acetylation, which may regulate its activity or stability.
• The gene undergoes alternative splicing, producing multiple isoforms that could have distinct functions.
• The three-dimensional structure of PHPT1 has been solved, providing insights into its catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen for this antibody covers the full-length human PHPT1 (amino acids 1–125), likely enabling recognition of native and denatured forms; consider this when designing assays.
• For Western blotting, validate the antibody using lysates from heart or skeletal muscle, where PHPT1 is highly expressed; a specific band near 14 kDa is expected.
• For ELISA applications, use recombinant PHPT1 protein for coating or as a standard, and confirm linearity and sensitivity in the relevant sample matrix.
• Given the reported cross-reactivity with mouse and rat, this antibody may be useful in rodent models; however, verify performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to accelerate diagnostic development. The PHPT1 rabbit polyclonal antibody supports research into phosphohistidine phosphatase biology, and CamelBio’s integrated supply chain can meet your full raw-material needs from discovery to commercialization.