Anti-PHOX2B Rabbit Polyclonal Antibody for WB, ELISA - Q99453

Core Product Specifications and Parameters

Biological Background: PHOX2B Function and Localization

• Encoded by the PHOX2B gene, also known as paired mesoderm homeobox protein 2B, NBPhox, and PMX2B, it functions as a transcription factor involved in the development of noradrenergic neuron populations, particularly the locus coeruleus.
• Enhances the transcriptional activity of the dopamine beta-hydroxylase and c-fos promoters through second-messenger-mediated pathways, contributing to neurotransmitter phenotype determination in vertebrates.
• Localizes to the nucleus where it binds DNA via its homeobox domain, regulating downstream target genes critical for autonomic nervous system development.
• Highly expressed in neuroblastoma tissues, brain, and adrenal gland, consistent with its role in neural crest-derived cell lineages.
• Belongs to the paired-like homeobox family of transcription factors, characterized by a conserved homeodomain important for DNA binding and transcriptional regulation.
• Key post-translational modifications include isopeptide bond formation and ubiquitin conjugation, which may influence protein stability or activity.
• Associated with disease variants linked to congenital central hypoventilation syndrome (CCHS) and neuroblastoma, underscoring its clinical relevance in autonomic dysfunction and cancer.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 260-314) of human PHOX2B, which may provide high specificity by avoiding the conserved homeodomain region present in other paired-like homeobox proteins.
• For Western blot, the antibody detects a band at approximately 32 kDa, consistent with the calculated molecular weight of 31.6 kDa for human PHOX2B. Use appropriate protein ladder for size verification.
• This polyclonal antibody is suitable for ELISA applications; consider performing a titration to optimize the dilution for your specific assay conditions.
• Cross-reactivity is reported with human and mouse PHOX2B; for other species, validate reactivity in the relevant sample system before use.
• Since PHOX2B is a transcription factor, nuclear extracts or whole cell lysates from neuroblastoma cell lines or brain tissue are recommended sample types for detection.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop platform for IVD raw materials, CamelBio connects diagnostic manufacturers and research laboratories with high-quality antibodies like this PHOX2B polyclonal antibody, supporting neuroblastoma and autonomic nervous system research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and efficient sourcing of rare targets to accelerate your assay development from concept to clinic.