Anti-PHOX2B Rabbit Polyclonal Antibody for WB and ELISA - Q99453

Core Product Specifications and Parameters

Biological Background: PHOX2B Function and Localization

• PHOX2B (Paired mesoderm homeobox protein 2B) is a paired-like homeobox transcription factor encoded by the PHOX2B gene, also known as NBPhox or PMX2B.
• It plays a critical role in the development of noradrenergic neuron populations, including the locus coeruleus, and may determine neurotransmitter phenotype.
• PHOX2B enhances second-messenger-mediated activation of the dopamine beta-hydroxylase and c-fos promoters via cAMP-response and serum-response elements.
• The protein localizes to the nucleus, consistent with its role as a transcription factor.
• Tissue expression is prominent in neuroblastoma, brain, and adrenal gland.
• Mutations in PHOX2B are associated with congenital central hypoventilation syndrome (CCHS) and neuroblastoma predisposition, often involving polyalanine tract expansions.
• PHOX2B contains a homeobox DNA-binding domain and is subject to post-translational modifications, including ubiquitination and isopeptide bond formation.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from neuroblastoma cell lines (e.g., SK-N-SH) or mouse/rat brain tissue, expecting a band around 32 kDa.
• Due to cross-reactivity with mouse and rat, validate the optimal working dilution in the intended species.
• ELISA may be used for quantitative detection; consider using recombinant PHOX2B protein as a standard.
• The immunogen corresponds to the N-terminal region; thus, the antibody may detect multiple isoforms or cleavage products; confirm specificity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing solution for diagnostic manufacturers and researchers, providing access to validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This PHOX2B polyclonal antibody is part of our extensive neurobiology and oncology portfolio, supporting neural crest development and neuroblastoma studies. Our team can assist with custom bulk orders, rare target sourcing, and technical consultation to accelerate your diagnostic development.