Anti-PHOX2B Rabbit Monoclonal Antibody for WB, ELISA - Q99453

Core Product Specifications and Parameters

Biological Background: PHOX2B Function and Localization

• PHOX2B, also known as NBPhox or paired-like homeobox 2B, is a transcription factor encoded by the PHOX2B gene and belongs to the paired-like homeobox family.
• It plays a critical role in the development of several major noradrenergic neuron populations, including the locus coeruleus, and helps determine neurotransmitter phenotypes in vertebrates.
• Functions as a transcriptional activator, enhancing second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, as well as cAMP-response element and serum-response element enhancers.
• Localizes exclusively to the nucleus, consistent with its function as a DNA-binding transcription factor that regulates gene expression.
• Primarily expressed in neuroblastoma, brain, and adrenal gland, aligning with its role in neural crest-derived tissues and neuroblastoma pathology.
• Mutations in PHOX2B are associated with congenital central hypoventilation syndrome (CCHS) and neuroblastoma susceptibility, underscoring its clinical significance.
• Contains a conserved homeobox domain and undergoes post-translational modifications such as isopeptide bond formation and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 215–314, a region near the homeodomain, which may contribute to specific detection of PHOX2B; validate in the relevant sample system.
• For WB, the predicted molecular weight is approximately 32 kDa; consider using appropriate positive controls (e.g., neuroblastoma cell lines) and loading controls to confirm specificity.
• For ELISA, the antibody may be used as a capture or detection reagent; optimization of coating concentration and pairing with a validated secondary antibody is recommended.
• Cross-reactivity with mouse implies sequence conservation across species; when using mouse models, verify reactivity in the specific tissue or cell type of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers with validated antibodies, bulk ancillary reagents, and specialized technical services from concept to clinic. For PHOX2B-related neurobiology and oncology studies, we offer optimized monoclonal antibodies, including this rabbit mAb, along with the capability to source rare-target raw materials and develop custom antibody pairs for your assay needs. Contact us to streamline your procurement and accelerate your project timelines with reliable, high-quality reagents.