Anti-Phospholipid Scramblase 1 (PLSCR1) Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15162

Core Product Specifications and Parameters

Biological Background: Phospholipid Scramblase 1 (PLSCR1) Function and Localization

• PLSCR1 is a calcium-dependent phospholipid scramblase that catalyzes ATP-independent bidirectional movement of phospholipids across the plasma membrane, leading to phosphatidylserine externalization and loss of membrane asymmetry. Related references: PMID:10770950 PMID:18629440 PMID:23590222
• Beyond scramblase activity, PLSCR1 exhibits magnesium-dependent nuclease activity against double‑stranded DNA and RNA, and can enhance DNA decatenation mediated by TOP2A. Related references: PMID:17567603 PMID:27206388
• It negatively regulates FcR‑mediated phagocytosis in differentiated macrophages. Related references: PMID:26745724
• PLSCR1 plays a critical role in the antiviral interferon response, inhibiting replication of multiple viruses including HIV‑1, hepatitis B, influenza A, and SARS‑CoV‑2 by targeting viral proteins or nascent viral vesicles. Related references: PMID:15308695 PMID:22789739 PMID:23501106 PMID:25365352 PMID:29352288 PMID:35595813 PMID:37438530
• It also functions as a host cell attachment receptor for hepatitis C virus (HCV).
• Subcellularly, PLSCR1 localizes to the cell membrane, nucleus, cytoplasm, and perinuclear region.
• Tissue expression is broad (platelets, erythrocytes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney, pancreas) but is notably absent from brain and skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1‑100 at the N‑terminus of human PLSCR1 (NP_066928.1). This region may be accessible in native conformations, making the antibody well‑suited for detection in a variety of assay formats.
• For Western blot (WB), a band around 35kDa is expected; consider using appropriate positive controls (e.g., human, mouse, or rat cell lysates) and optimizing blocking and washing conditions to reduce non‑specific binding.
• In IHC‑P and IF/ICC, validate the antibody’s performance with both positive and negative tissue/cell controls, as PLSCR1 expression can vary. Antigen retrieval methods and permeabilization steps may influence the staining pattern.
• ELISA applications may be developed using recombinant PLSCR1 or native protein; check cross‑reactivity with related family members to ensure specificity.
• Since cross‑reactivity is reported for human, mouse, and rat, verify the antibody’s reactivity in the chosen species with proper loading controls and, if necessary, species‑adapted secondary detection reagents.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is your trusted one‑stop sourcing partner for IVD raw materials, providing comprehensive access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare‑target sourcing. For research involving phospholipid scramblase 1 (PLSCR1)—covering areas such as apoptosis, phospholipid transport, and antiviral defense—our PLSCR1 rabbit monoclonal antibody serves as a reliable tool to support your diagnostic and research workflows.