Anti-Phospholamban (PLN) Polyclonal Antibody for WB, ELISA - P26678

Core Product Specifications and Parameters

Biological Background: Phospholamban Function and Localization

• Phospholamban (PLN), also known as PLB, is a 52-amino acid transmembrane protein that reversibly inhibits the sarcoplasmic reticulum Ca2+-ATPase (SERCA2) in cardiac muscle by reducing the pump's apparent affinity for calcium, thereby regulating cardiac contractility (PubMed:28890335). Related references: PMID:28890335
• PLN preferentially binds to the ATP-bound E1 conformational state of SERCA2, which predominates during diastolic low Ca2+ levels, and disrupts the allosteric activation of the pump by ATP.
• The inhibitory function of PLN is modulated by its oligomeric state and is relieved upon phosphorylation, linking beta-adrenergic stimulation to enhanced cardiac relaxation.
• Beyond cardiac SERCA2, PLN also inhibits the activity of ATP2A3/SERCA3, extending its regulatory role to other tissues and processes such as germ cell differentiation and calcium homeostasis in elongated spermatids.
• In the brain, PLN is expressed in the thalamic reticular nucleus where it contributes to the regulation of sleep patterns and executive functions.
• PLN is primarily localized to the membranes of the sarcoplasmic/endoplasmic reticulum and mitochondria in heart muscle, with additional expression in smooth muscle and other tissues at lower levels.
• Clinically, mutations in the PLN gene are associated with cardiomyopathies, highlighting its critical role in cardiac physiology and disease.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (residues 1-52). For Western blot (WB), ensure that the detection system is optimised for small proteins (approx. 6 kDa monomer) – consider using low‑molecular‑weight markers, PVDF membranes, and short transfer times.
• For ELISA, the peptide immunogen can serve as a positive control; validate the assay with relevant tissue lysates or recombinant PLN.
• Given the cross‑reactivity with mouse and rat, this antibody may be suitable for studies in rodent cardiac tissue; however, verify species‑specific reactivity in your own experimental system.
• Because PLN function is phosphorylation‑dependent, consider phosphatase inhibitors in lysis buffers when assessing the protein’s biological state via WB.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is a trusted one‑stop partner for IVD raw material procurement, supporting diagnostic manufacturers and research laboratories with high‑quality antibodies and reagents. This anti‑PLN polyclonal antibody is ideal for studies of cardiac calcium handling and cardiomyopathy. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare target raw materials to accelerate your projects from concept to clinic.