Anti-Phospho-ZAP70 (Y493) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P43403

Core Product Specifications and Parameters

Biological Background: ZAP70 Function and Localization

• ZAP70 (70 kDa zeta-chain associated protein) is a cytoplasmic tyrosine kinase essential for adaptive immune regulation, mediating T-cell receptor (TCR) signaling upon antigen presentation (PubMed:7509083). Related references: PMID:7509083
• The protein contains tandem SH2 domains that bind phosphorylated ITAM motifs on CD3 chains, relieving autoinhibition and enabling LCK-mediated phosphorylation at Tyr493 for full activation.
• Upon activation, ZAP70 phosphorylates key adaptors LAT and LCP2, triggering downstream cascades that regulate T-cell proliferation, cytokine production, and cytoskeletal reorganization.
• ZAP70 is involved in thymocyte development, promoting survival and cell-cycle progression of CD4/CD8 double-negative cells before positive selection.
• It also contributes to B-cell development and activation through B-cell receptor signaling, highlighting broader lymphoid roles.
• Subcellular localization is primarily cytoplasmic, with recruitment to the plasma membrane upon TCR engagement; it also participates in TCR internalization via CBL-mediated ubiquitination.
• Expressed predominantly in T-cells, natural killer cells, early thymocytes, and pro/pre B-cells, with genetic defects causing severe combined immunodeficiency (SCID) and autoimmune conditions.
• Post-translational modifications include tyrosine phosphorylation, acetylation, and ubiquitination, critical for its dynamic regulation.

Experimental Guidance and Technical Tips

• As a phospho-specific antibody targeting Tyr493, ensure samples are treated with phosphatase inhibitors during lysis and consider phosphatase treatment as a negative control.
• For Western blot, the predicted molecular weight is approximately 70 kDa; use an appropriate gel percentage (e.g., 8-10%) for optimal resolution.
• Validate antibody specificity in your model system using peptide competition or genetic knockdown/knockout approaches.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; test pH 6 or pH 9 retrieval buffers.
• In immunofluorescence (IF/ICC), monitor membrane localization upon stimulation; permeabilization and fixation conditions should be optimized.
• ELISA and other applications may require titration to determine the optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic and research communities from concept to clinic. This anti-phospho-ZAP70 (Tyr493) antibody, targeting a key node in adaptive immunity and T-cell signaling, exemplifies our capability to supply validated phospho-specific antibodies for demanding applications. We also offer comprehensive antibody development services, bulk reagent supply, and rare target sourcing to streamline your project workflows.