Anti-Phospho-YWHAZ-S58 Rabbit pAb for WB and IF/ICC - P63104

Core Product Specifications and Parameters

Biological Background: YWHAZ (14-3-3 zeta/delta) Function and Localization

• YWHAZ (14-3-3 protein zeta/delta) serves as a pivotal adapter protein that integrates diverse signaling pathways by recognizing phosphoserine or phosphothreonine motifs on binding partners. Related references: PMID:14578935 PMID:15071501 PMID:15644438
• It promotes cytosolic retention and inactivation of TFEB transcription factor through phosphorylation-dependent binding. Related references: PMID:35662396
• YWHAZ stimulates ARHGEF7 activity on RAC1, leading to enhanced lamellipodia and membrane ruffle formation. Related references: PMID:16959763
• In neurons, YWHAZ regulates spine maturation via modulation of ARHGEF7 activity (By similarity).
• Subcellular localization is primarily cytoplasmic, with additional presence in melanosomes.
• The protein undergoes various post-translational modifications, including phosphorylation and acetylation, and is subject to alternative splicing.
• YWHAZ is associated with disease variants linked to intellectual disability, underscoring its role in neuronal development and function.

Experimental Guidance and Technical Tips

• For western blotting, consider including a phospho‑specific control and probing total YWHAZ levels to confirm phosphorylation specificity.
• For immunofluorescence, optimize fixation and permeabilization conditions; methanol fixation may help preserve the phospho‑epitope.
• Validate antibody reactivity in your specific cell line or tissue, as endogenous phosphorylation levels can vary.
• Use phosphatase inhibitors during sample preparation to maintain the phospho signal.

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