Anti-Phospho-YAP1-S128 Rabbit Polyclonal Antibody for WB - P46937

Core Product Specifications and Parameters

Biological Background: Transcriptional coactivator YAP1 Function and Localization

• YAP1 (Yes-associated protein 1) functions as a transcriptional coactivator and corepressor, serving as a critical downstream effector of the Hippo signaling pathway that regulates organ size, cell proliferation, and apoptosis; its dysregulation is linked to tumorigenesis. Related references: PMID:17974916, PMID:18280240, PMID:18579750
• In the Hippo kinase cascade, STK3/MST2 and STK4/MST1 phosphorylate and activate LATS1/2, which in turn phosphorylate YAP1 at multiple residues including Ser128, promoting its cytoplasmic retention by 14-3-3 and preventing nuclear coactivation of TEAD transcription factors. Related references: PMID:18158288, PMID:26598551, PMID:34404733
• Upon nuclear translocation, YAP1 binds TEAD family transcription factors to activate genes driving cell growth, anchorage-independent growth, and epithelial-mesenchymal transition (EMT), contributing to cancer progression. Related references: PMID:18579750
• YAP1 is predominantly cytoplasmic but shuttles to the nucleus; it also localizes to tight junctions and the cell membrane, consistent with its role in contact inhibition and tissue architecture.
• Beyond Hippo signaling, YAP1 regulates the cortical actomyosin network via ARHGAP18 to control tissue tension and 3D shape, and acts as a transcriptional corepressor of TEAD4 to suppress ciliogenesis. Related references: PMID:25778702, PMID:25849865
• Isoforms of YAP1 lacking the transactivation domain are observed in striatal neurons of Huntington disease patients, indicating tissue-specific alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide phosphorylated at Ser128. For best results, consider shipping and storing under low-temperature conditions (e.g., ice bag during transport) to maintain stability of the phospho-specific antibody.
• This antibody is suitable for Western blotting (predicted band at ~54 kDa) and ELISA. Validate specificity in your experimental system by comparing signals from phosphatase-treated lysates or using non-phosphorylated peptide competition.
• Cross-reactivity with human and mouse YAP1 allows for comparative studies; confirm reactivity in the relevant sample system before large-scale experiments.

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