Anti-Phospho-YAP1-S127 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P46937

Core Product Specifications and Parameters

Biological Background: YAP1 Function and Localization

• Transcriptional regulator YAP1 (Yes-associated protein 1, Yorkie homolog) is a core component of the Hippo signaling pathway, where it acts as both a coactivator and corepressor to control organ size and suppress tumorigenesis. Related references: PMID:17974916 PMID:18280240
• LATS1/2-mediated phosphorylation at Ser127 promotes YAP1 cytoplasmic retention by generating a 14-3-3 binding site, thereby inhibiting its nuclear translocation and transcriptional activity. Related references: PMID:18158288 PMID:26598551 PMID:34404733
• Nuclear YAP1 binds TEAD transcription factors to regulate genes involved in cell proliferation, anchorage-independent growth, and epithelial-mesenchymal transition (EMT). Related references: PMID:18579750
• YAP1 regulates 3D tissue morphology and actomyosin contractility via ARHGAP18, and acts as a corepressor to suppress ciliogenesis by downregulating AURKA and PLK1 expression. Related references: PMID:25778702 PMID:25849865
• YAP1 cooperates with WWTR1/TAZ to modulate TGF-β/SMAD signaling and synergizes with WBP2 to enhance progesterone receptor transcriptional activity. Related references: PMID:16772533
• YAP1 localizes predominantly to the cytoplasm and translocates to the nucleus upon activation; it is also found at tight junctions and the cell membrane.
• Increased YAP1 expression is associated with liver and prostate cancers, while N-terminally truncated isoforms are detected in Huntington disease striatal neurons.
• YAP1 is classified as a phosphoprotein and proto-oncogene, reflecting its phosphorylation-dependent regulation and oncogenic potential.

Experimental Guidance and Technical Tips

• For phospho-specific detection, always include phosphatase inhibitor cocktails during cell lysis and sample preparation to preserve Ser127 phosphorylation status.
• Verify phospho-specificity by treating samples with lambda phosphatase or calf intestinal phosphatase before immunoblotting.
• For IHC-P, optimize antigen retrieval methods (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) to balance phospho-epitope exposure and tissue morphology.
• For IF/ICC, permeabilize cells with 0.1–0.5% Triton X-100 and use a blocking buffer compatible with phospho-antibody detection (e.g., 5% BSA in TBST).
• When performing ELISA, consider using a non-phosphorylated peptide as a negative control to validate specificity.
• Since this is a rabbit polyclonal, use an appropriate anti-rabbit secondary antibody and confirm optimal dilutions empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop bridge to premium IVD raw materials, offering validated antibodies, including phospho-specific reagents like this anti-Phospho-YAP1-S127 antibody, to support cancer biomarker research and Hippo signaling studies. Our comprehensive portfolio includes matched antibody pairs, bulk monoclonals and polyclonals, and custom sourcing for rare targets, backed by technical and regulatory consulting. Get in touch to accelerate your diagnostic development from concept to clinic.