Anti-Phospho-YAP1-S127 Rabbit Monoclonal Antibody for WB, ELISA - P46937

Core Product Specifications and Parameters

Biological Background: YAP1 Function and Localization

• YAP1 is a transcriptional coactivator and corepressor that serves as a critical downstream effector of the Hippo signaling pathway, regulating organ size, proliferation, and apoptosis.
  Related references: PMID:17974916, PMID:18280240, PMID:18579750

• In the canonical Hippo pathway, LATS1/2 kinases phosphorylate YAP1 at S127, promoting cytoplasmic retention and preventing nuclear translocation, thereby inhibiting its transcriptional activity.
  Related references: PMID:18158288, PMID:26598551, PMID:34404733

• YAP1 partners with TEAD transcription factors to drive expression of genes involved in cell growth, anchorage independence, and epithelial-mesenchymal transition (EMT).
  Related references: PMID:18579750

• It modulates tissue tension and 3D morphology by suppressing F-actin polymerization through ARHGAP18, a Rho GTPase-activating protein.
  Related references: PMID:25778702

• YAP1 acts as a transcriptional corepressor of TEAD4 target genes AURKA and PLK1 to suppress ciliogenesis.
  Related references: PMID:25849865

• Subcellularly, YAP1 is found in the cytoplasm and nucleus, and also associates with tight junctions and the cell membrane.

• Tissue specificity: Elevated expression in some liver and prostate cancers; isoforms lacking the transactivation domain are noted in striatal neurons of Huntington disease patients (at protein level).

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phosphopeptide corresponding to the S127 phosphorylation site of human YAP1. Cross-reactivity with the unphosphorylated protein should be carefully evaluated in experimental systems.
• For Western blot, consider validating the phospho-specificity by treating samples with phosphatase inhibitors or comparing to dephosphorylated controls.
• The product has been validated for WB and ELISA in human, mouse, and rat samples. Optimize primary antibody dilution and detection conditions according to your specific assay requirements.
• Depending on the sample, multiple bands corresponding to phosphorylated YAP1 isoforms and post-translational modifications may be observed; molecular weight markers in the 36–54 kDa range are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with reliable one-stop access to validated IVD raw materials and rare-target reagents. This phospho-specific YAP1 (S127) rabbit monoclonal antibody supports Hippo pathway and cancer research applications, and is part of our broader portfolio of phosphorylation-state-specific antibodies, bulk ancillary reagents, and custom antibody development services. Contact our team to discuss your specific procurement or technical requirements.