Anti-Phospho-Vimentin-S83 Rabbit Polyclonal Antibody for WB, ELISA - P08670

Core Product Specifications and Parameters

Biological Background: Vimentin Function and Localization

• Vimentin is a 54 kDa class-III intermediate filament protein encoded by the VIM gene (Gene ID: 7431) and is a member of the intermediate filament family. It is alternatively known as CTRCT30 or HEL113.
• It forms cytoskeletal networks predominantly in mesenchymal cells and plays a critical role in maintaining cell shape, integrity, and mechanical support.
• Vimentin is involved in cell migration, directional movement, cell sheet organization, and Golgi complex polarization at the migration front, and it may promote axon outgrowth and motor fiber repair via DSP-mediated recruitment.
• It interacts with LARP6 to stabilize type I collagen mRNAs (COL1A1 and COL1A2), highlighting a role in extracellular matrix regulation.
• Subcellularly, vimentin localizes to the cytoplasm, cytoskeleton, nucleus matrix, cell membrane, and cell projections such as axons, associating with organelles including the nucleus, endoplasmic reticulum, and mitochondria.
• It is highly expressed in fibroblasts, with some expression in T- and B-lymphocytes, and is often overexpressed in hormone-independent mammary carcinoma cell lines, serving as a marker for epithelial-mesenchymal transition (EMT).
• Vimentin undergoes various post-translational modifications, including phosphorylation at Ser83 (this product’s target site), acetylation, glycation, S-nitrosylation, and ubiquitin conjugation, which regulate filament assembly and disassembly.
• It is implicated in diseases such as cataract and is known to interact with viral proteins (host-virus interaction), reflecting its broad functional relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser83 of human vimentin. When using this polyclonal antibody for Western blotting, consider that phosphorylation at Ser83 may be influenced by cellular signaling pathways such as those involving protein kinase C or growth factors.
• For ELISA applications, ensure the antigen presents the phosphorylated epitope in a conformation compatible with polyclonal recognition, and validate the detection in your specific sample matrix.
• Vimentin is highly expressed in mesenchymal cells and certain cancer cell lines; therefore, positive controls such as fibroblasts or hormone-independent mammary carcinoma cell lines (e.g., MDA-MB-231) may be appropriate for initial experiments.
• Rat cross-reactivity is predicted based on sequence homology; it is advisable to confirm reactivity in rat tissues or cell lines under your experimental conditions.
• The predicted molecular weight of vimentin is ~54 kDa, but phosphorylation and other modifications may cause slight shifts; verify the band pattern using appropriate molecular weight markers and phosphatase-treated controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges your IVD raw material needs with a one-stop portfolio of validated antibodies, bulk reagents, and customized rare-target sourcing. Focusing on cytoskeleton and EMT research, our offerings include phospho-specific antibodies like anti-phospho-Vimentin-S83 to support your diagnostic development. From antibody pairs to technical consulting, we deliver integrated solutions from concept to clinic.