Anti-Phospho-VEGF Receptor 2-Y1175 Rabbit Polyclonal Antibody for WB, ELISA - P35968

Core Product Specifications and Parameters

Biological Background: VEGFR2 (KDR) Function and Localization

• Vascular endothelial growth factor receptor 2 (VEGFR2), also designated KDR, FLK-1, or CD309, is a receptor tyrosine kinase that serves as the primary mediator of VEGF-induced endothelial signaling.
• VEGFR2 binds VEGFA, VEGFC, and VEGFD, playing an essential role in angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis.
• The full-length isoform 1 is a transmembrane protein with an extracellular ligand-binding domain and an intracellular kinase domain. Isoforms 2 and 3 lack a transmembrane domain and may function as soluble decoy receptors, with isoform 2 acting as a negative regulator of lymphangiogenesis.
• VEGFR2 is localized at the cell membrane, but also detected at cell junctions, within the endoplasmic reticulum, cytoplasm, nucleus, cytoplasmic vesicles, early endosomes, and as a secreted form.
• Upon ligand binding, VEGFR2 activates downstream signaling cascades including PLCG1, MAPK/ERK, and AKT pathways, promoting endothelial cell proliferation, survival, migration, and actin cytoskeleton reorganization.
• Phosphorylation at tyrosine 1175 (Y1175) is a critical event for VEGFR2 activation and subsequent recruitment of signaling adaptors such as PLCG1.
• VEGFR2 is widely expressed and has been detected at the protein level in the cornea. It is subject to post-translational modifications including phosphorylation, glycosylation, and ubiquitination.
• Keywords associated with VEGFR2 include tyrosine-protein kinase, angiogenesis, developmental protein, and receptor, reflecting its pivotal role in vascular biology and disease.

Experimental Guidance and Technical Tips

• The immunogen was designed as a synthetic peptide phosphorylated at Y1175; therefore, antibody specificity for the phospho-epitope should be confirmed using peptide competition assays or phosphatase treatment of samples.
• For Western blotting, a band corresponding to VEGFR2 at approximately 152 kDa is anticipated. Run appropriate molecular weight markers and consider using 6-8% SDS-PAGE gels with extended transfer times.
• This antibody is listed for ELISA; optimize coating antigen concentration, antibody dilution, and detection conditions for each assay system.
• Cross-reactivity is reported for human, mouse, and rat; validate performance in your specific species and tissue context, as phosphorylation status may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, to accelerate diagnostic assay development. For angiogenesis and cancer biomarker research, our one-stop sourcing service provides access to phospho-specific antibodies like anti-VEGFR2 pY1175 alongside complementary antigens and enzymes. Partner with CamelBio to streamline procurement and reduce time-to-clinic for your next-generation diagnostic kits.