Anti-Phospho-VASP (S157) Rabbit Monoclonal Antibody for WB, ELISA - P50552

Core Product Specifications and Parameters

Biological Background: Vasodilator-stimulated phosphoprotein Function and Localization

• VASP (Vasodilator-stimulated phosphoprotein) is an actin-associated protein encoded by the VASP gene (GeneID: 7408), belonging to the Ena/VASP family.
• It promotes actin filament elongation by protecting barbed ends from capping and facilitating the transfer of profilin-bound actin monomers, thereby enhancing polymerization.
• VASP plays critical roles in cytoskeleton-dependent processes including axon guidance, lamellipodial and filopodial dynamics, and cell migration.
• In platelets, VASP regulates actin dynamics and platelet aggregation, with high expression levels observed in platelets.
• The protein localizes to cytoplasmic sites of active actin remodeling such as focal adhesions, lamellipodia, filopodia, and tight junctions.
• Post-translational modifications, particularly phosphorylation at serine 157 (S157), modulate VASP activity and subcellular distribution.
• As a key phosphoprotein and actin regulator, VASP is implicated in vascular biology, hemostasis, and cancer cell invasion.

Experimental Guidance and Technical Tips

• Because the immunogen is a synthetic phosphopeptide around S157, the antibody is expected to specifically recognize the phosphorylated form of VASP. Use phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For Western blot, validate the antibody’s specificity by comparing untreated versus phosphorylated controls, or after phosphatase treatment of lysates.
• The reported cross-reactivity is limited to human samples; test in relevant human cell lines or platelet lysates.
• For ELISA applications, consider coating the synthetic phosphopeptide directly or employing a sandwich format with appropriate capture/detection pairs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers, researchers, and laboratories with a comprehensive portfolio of IVD raw materials, technical support, and sourcing solutions. This anti-Phospho-VASP (S157) antibody enables targeted studies on actin dynamics, platelet function, and cell motility. Beyond this product, CamelBio offers validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development from concept to clinic.