Anti-Phospho-VANGL2 (S79/S82/S84) Rabbit Monoclonal Antibody for WB and ELISA - Q9ULK5

Core Product Specifications and Parameters

Biological Background: VANGL2 Function and Localization

• VANGL2 (Vang-like protein 2) is a multi-pass transmembrane protein, also known as Loop-tail protein 1 homolog (LPP1, LTAP) or Strabismus 1 (STB1).
• It plays a critical role in early morphogenesis and patterning of axial midline structures and neural plate development.
• VANGL2 is a key regulator of planar cell polarity (PCP), essential for the proper orientation of stereociliary bundles in the cochlea and the polarization and movement of myocardializing cells in the outflow tract via RHOA signaling.
• It functions in the cell membrane and is required for the cell surface localization of frizzled receptors FZD3 and FZD6 in the inner ear.
• This antibody specifically recognizes VANGL2 phosphorylated at serines 79, 82, and 84, enabling the study of phosphorylation-dependent regulation in developmental processes.
• Clinically, VANGL2 mutations are associated with neural tube defects, underscoring its importance in human development.

Experimental Guidance and Technical Tips

• Because this antibody targets phosphorylation sites, ensure that samples are prepared with phosphatase inhibitors to preserve the phosphorylated epitopes for Western blotting.
• For ELISA applications, consider coating with a phospho-peptide corresponding to the S79/S82/S84 region to assess binding specificity, and include non-phosphorylated peptide controls to verify phospho-selectivity.
• Validate antibody performance in your specific cell type or tissue extract, as endogenous VANGL2 expression levels may vary; human and rat samples are known to cross-react.
• When performing Western blotting, the predicted molecular weight of VANGL2 is approximately 60 kDa; however, post-translational modifications may cause slight migration shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with a comprehensive one-stop supply of IVD raw materials, technical services, and consulting—from concept to clinic. For planar cell polarity and developmental biology research, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, including this anti-phospho-VANGL2 antibody. Our sourcing capabilities also extend to rare-target raw materials, ensuring seamless integration into your assay development pipeline.