Anti-Phospho-β-Catenin (S552) Polyclonal Antibody for WB, ELISA - P35222

Core Product Specifications and Parameters

Biological Background: β-Catenin (CTNNB1) Function and Localization

• Acts as a key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a destruction complex with AXIN1, APC, and GSK3B that phosphorylates N-terminal Ser/Thr residues, leading to ubiquitination and proteasomal degradation. Upon Wnt stimulation, accumulates in the nucleus and serves as a coactivator for TCF/LEF transcription factors to activate Wnt-responsive genes. Related references: PMID:17524503, PMID:18077326, PMID:18086858
• Also acts as a transcriptional coactivator for other factors such as NR5A2, expanding its regulatory roles beyond the canonical TCF/LEF pathway. Related references: PMID:22187462
• Promotes epithelial to mesenchymal transition (EMT) and mesenchymal to epithelial transition (MET) by driving transcription of CTNNB1/TCF-target genes, linking Wnt signaling to developmental and cancer processes. Related references: PMID:29910125
• Functions as a cell adhesion molecule by binding to E-cadherin at adherens junctions, thereby coupling cadherin-mediated adhesion to the actin cytoskeleton.
• Participates in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway that mediates insulin internalization. Related references: PMID:21262353
• Blocks anoikis in malignant kidney and intestinal epithelial cells by down-regulating DAPK2, promoting anchorage-independent growth. Related references: PMID:18957423
• Disrupts PML nuclear body formation by inhibiting RANBP2-mediated sumoylation of PML. Related references: PMID:22155184
• Widely localized within the cell, found in the cytoplasm, nucleus, adherens junctions, centrosome, spindle poles, and cell membrane. Highly expressed in hair follicle cells, colon, and cortical neurons, with elevated protein levels reported in breast cancer tissues. Related references: PMID:29367600

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S552 of human β-Catenin. When performing western blot (WB), verify that the detected band corresponds to the expected 85 kDa protein and is specifically enriched under conditions that promote S552 phosphorylation (e.g., Wnt pathway activation).
• For ELISA applications, consider pairing this detection antibody with a capture antibody that recognizes total β-Catenin to measure phospho-S552 levels; optimize coating and detection conditions using phosphorylated and non-phosphorylated peptide controls.
• Given cross-reactivity with human, mouse, and rat, validate the antibody's performance in your specific species and tissue/cell line model, as phosphorylation patterns and epitope accessibility may vary.
• Include phosphatase inhibitors in lysis buffers during sample preparation to preserve the phosphorylation signal.
• Assess antibody specificity by comparing signals in untreated versus phosphatase-treated lysates or by using a non-phospho peptide blocking control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic development. With expertise in phospho-specific antibodies and Wnt signaling targets, we provide reliable sourcing support for rare and specialized reagents like this anti-phospho-β-Catenin (S552) antibody, helping you streamline your workflow from discovery to clinical application.