Anti-Phospho-Ubiquitin-Ser65 Rabbit Polyclonal Antibody for WB, ELISA - P62979 / P62987 / P0CG47 / P0CG48

Core Product Specifications and Parameters

Biological Background: Ubiquitin Function and Localization

• Ubiquitin is a highly conserved 76-amino acid protein expressed from four genes (RPS27A, UBA52, UBB, UBC), yielding fusion proteins (e.g., ubiquitin-ribosomal protein eS31 and eL40) and polyubiquitin precursors.
• It acts as a post-translational modifier by covalent conjugation to lysine residues of target proteins, forming monomeric or polyubiquitin chains. Different linkage types (Lys-6, -11, -29, -33, -48, -63 and linear) dictate diverse cellular outcomes, including proteasomal degradation (Lys-48), DNA repair (Lys-6/63), endocytosis and NF-κB signaling (Lys-63).
• As a fusion with ribosomal protein eS31, ubiquitin is integral to the 40S ribosomal subunit and essential for small subunit processome assembly and ribosome biogenesis. Related references: PMID:23636399 PMID:9582194 PMID:34516797
• Ubiquitin localizes predominantly in the cytoplasm and nucleus, including the nucleolus, consistent with its roles in protein degradation, signaling, and ribosome biogenesis.
• Post-translational modifications, including phosphorylation at Ser65, acetylation, and ADP-ribosylation, regulate ubiquitin's structure and function. Phosphorylation at Ser65 specifically modulates ubiquitin chain assembly and is implicated in mitophagy and innate immune signaling.

Experimental Guidance and Technical Tips

• For Western blot, consider using phosphatase inhibitors in lysis buffers to preserve phosphorylation at Ser65.
• Validate phospho-specificity by treating samples with lambda phosphatase; the signal should disappear upon dephosphorylation.
• Include appropriate positive and negative controls (e.g., stimulated vs. unstimulated cells) to confirm antibody specificity.
• For ELISA, optimize coating antigen concentration and antibody dilution to achieve optimal signal-to-noise ratio.
• Note that the antibody may recognize ubiquitin from all four genes due to conserved sequence around Ser65; consider using gene-specific knockdowns if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing validated antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic development from concept to clinic. The Phospho-Ubiquitin-Ser65 antibody is a valuable tool for studying ubiquitination and phosphorylation-dependent signaling pathways. Explore our comprehensive catalog for optimized antibody pairs, monoclonal and polyclonal antibodies, and customized raw-material solutions for your research and diagnostic needs.