Anti-Phospho-TIFA-T9 Rabbit Monoclonal Antibody for WB, ELISA - Q96CG3

Core Product Specifications and Parameters

Biological Background: TRAF-interacting protein with FHA domain-containing protein A Function and Localization

• TRAF-interacting protein with FHA domain-containing protein A (TIFA), also known as T2BP, T6BP, or TIFAA, is encoded by the TIFA gene (Gene ID: 92610) and belongs to a family of proteins involved in innate immune signaling.
• Adapter molecule that plays a key role in the activation of pro-inflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs). Related references: PMID:12566447, PMID:15492226, PMID:26068852
• Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. Related references: PMID:12566447, PMID:15492226, PMID:26068852
• TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of pro-inflammatory NF-kappa-B signaling. Related references: PMID:30111836
• Localizes predominantly to the cytoplasm, where it can interact with upstream kinases and downstream effectors such as TRAF6.
• Post-translational phosphorylation at Thr-9 is a critical regulatory event, converting TIFA into an active signaling platform; additional phosphorylation sites may exist as indicated by its classification as a phosphoprotein.
• The protein has a molecular weight of approximately 21.4 kDa (calculated from sequence) and is part of a network linking pathogen sensing to inflammatory responses.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr-9 of human TIFA, suggesting the antibody is designed to specifically recognize the Thr-9 phosphorylated form of TIFA. Confirm specificity using dephosphorylated controls or relevant inhibitors.
• For Western blotting, predicted band size is ~21 kDa; consider optimizing blocking and detection conditions to minimize non-specific binding.
• In ELISA, ensure the peptide or protein antigen is properly coated and that the phosphorylation state is preserved during sample preparation.
• Since cross-reactivity is stated only for human, validate the antibody in the appropriate human cell lines or tissue samples where TIFA phosphorylation is induced (e.g., upon ALPK1 activation by ADP-Heptose).
• This antibody is suitable for applications such as WB and ELISA; for other assay formats, pilot experiments are recommended to verify performance.

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