Anti-Phospho-TDP-43/TARDBP (S409/S410) Rabbit Polyclonal Antibody for WB, DB, ELISA - Q13148

Core Product Specifications and Parameters

Biological Background: TDP-43 Function and Localization

• TDP-43 is an RNA-binding protein that preferentially binds GU-repeats via its RRM1 and RRM2 motifs, regulating diverse RNA processing events (PubMed:23519609, 24240615, 24464995). Related references: PMID:23519609 PMID:24240615 PMID:24464995
• It plays critical roles in splicing regulation of neuronal survival genes and mRNAs implicated in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) (PubMed:21358640, 29438978). Related references: PMID:21358640 PMID:29438978
• TDP-43 localizes predominantly to the nucleus but translocates to the cytoplasm and stress granules under oxidative stress, contributing to cell survival (PubMed:19765185, 23398327). Related references: PMID:19765185 PMID:23398327
• It maintains mitochondrial homeostasis by processing mitochondrial transcripts (PubMed:28794432). Related references: PMID:28794432
• TDP-43 regulates mRNA stability by recruiting CNOT7 deadenylase, promoting poly(A) tail shortening (PubMed:30520513). Related references: PMID:30520513
• As a phosphoprotein, TDP-43 undergoes phosphorylation at multiple residues, with S409/S410 phosphorylation linked to pathological aggregation in ALS and related disorders.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around S409/S410; antibody specificity should be confirmed by phosphatase treatment or competitive assays with non-phosphorylated peptide.
• For western blotting, expect a band near 45 kDa. Phosphorylation-dependent mobility shifts may occur; consider running parallel samples treated with lambda phosphatase.
• This antibody is suitable for DB and ELISA to detect phosphorylated TDP-43. Use appropriate positive controls (e.g., stressed cell lysates) and validate in the relevant sample system.

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